Comprehensive analysis and expression profiles of cassava UDP-glycosyltransferases (UGT) family reveal their involvement in development and stress responses in cassava
Comprehensive analysis and expression profiles of cassava UDP-glycosyltransferases (UGT) family reveal their involvement in development and stress responses in cassava
复制标题
木薯 UDP-糖基转移酶 (UGT) 家族的综合分析和表达谱揭示了它们参与木薯的发育和应激反应
DOI：
10.1016/j.ygeno.2021.08.004
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发表时间：
2021-08-18
期刊：
影响因子：
4.4
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Wu, Chunlai;Dai, Jing;Hu, Wei
UDP-糖基转移酶（UGT）广泛参与植物生长和逆境响应。然而，UGT家族在木薯中的研究还不多。本研究共鉴定了121个MeUGT基因，并通过系统发育分析将其分为14个亚家族。所有MeUGT蛋白都具有UGT家族的典型特征。串联复制是MeUGT家族扩张的关键驱动力。顺式作用元件分析揭示了14种与生物和非生物胁迫反应相关的顺式作用元件。转录组学和qRT-PCR分析表明，MeUGT基因参与了采后贮藏根的生理劣变以及对生物和非生物胁迫的响应。其中MeUGT-14/41在Xam处理后显著诱导。MeUGT-14或MeUGT-41的沉默降低了木薯对Xam的抗性，验证了转录组学数据用于功能预测的准确性。本研究揭示了MeUGTs家族的功能，为MeUGTs相关木薯遗传改良奠定了基础。
UDP-glycosyltransferases (UGTs) are widely involved in plant growth and stress responses. However, UGT family are not well understood in cassava. Here, we identified 121 MeUGT genes and classified them into 14 subfamilies by phylogenetic analysis. All MeUGT proteins have typical feature of the UGTs family. Tandem duplications are the crucial driving force for the expansion of MeUGT family. Cis-Acting elements analysis uncovered those 14 kinds of cis-elements associated with biotic and abiotic stress responses. Transcriptomic and qRT-PCR analyses indicated that MeUGT genes participate in postharvest physiological deterioration of storage root and the responses of biotic and abiotic stresses. Of which, MeUGT-14/41 were significantly induced after Xam treatment. Silencing of MeUGT-14 or MeUGT-41 reduced cassava resistance to Xam, verifying the accuracy of transcriptomic data for function prediction. Together, this study characterized the MeUGTs family and revealed their potential functions, which build a solid foundation for MeUGTs associated genetic improvement of cassava.