Callose and homogalacturonan epitope distribution in stomatal complexes of Zea mays and Vigna sinensis
Callose and homogalacturonan epitope distribution in stomatal complexes of Zea mays and Vigna sinensis
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DOI：
10.1007/s00709-019-01425-8
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发表时间：
2020-01-01
期刊：
影响因子：
2.9
通讯作者：
Apostolakos, P.
中科院分区：
文献类型：
--
作者：
Giannoutsou, E.;Sotiriou, P.;Apostolakos, P.
本文论述了胼胝质和同型半乳糖醛酸（HG）抗原决定簇的分布识别LM 20，JIM 5，和2F4抗体在细胞壁的分化和功能的气孔复合体的单子叶植物玉米和双子叶植物豇豆。结果表明，在气孔发育过程中，在这些植物种，胼胝质出现在一个精确的空间和时间控制的方式在保卫细胞（GC）的细胞壁。在这两种植物的功能气孔，胼胝质构成了一个占主导地位的细胞壁基质材料的极性腹侧细胞壁端和当地GC细胞壁加厚。在玉米中，LM 20、JIM 5或2F4抗体识别的HG表位主要位于气孔复合体的扩展细胞壁区域，而在豇豆中，它们沉积在GC的局部细胞壁增厚处以及气孔的壁架上。考虑到目前的数据有利于认为，在气孔复合体的单子叶植物Z。在玉米和双子叶植物中，酯化的HGs有助于GC形态发生过程中细胞壁的扩张和气孔的开放。此外，胼胝质和高度去酯化的HGs允许GC细胞壁区域承受气孔功能期间施加的机械应力。
This article deals with the distribution of callose and of the homogalacturonan (HG) epitopes recognized by LM20, JIM5, and 2F4 antibodies in cell walls of differentiating and functioning stomatal complexes of the monocotyledon Zea mays and the dicotyledon Vigna sinensis. The findings revealed that, during stomatal development, in these plant species, callose appears in an accurately spatially and timely controlled manner in cell walls of the guard cells (GCs). In functioning stomata of both plants, callose constitutes a dominant cell wall matrix material of the polar ventral cell wall ends and of the local GC cell wall thickenings. In Zea mays, the LM20, JIM5, or 2F4 antibody-recognized HG epitopes were mainly located in the expanding cell wall regions of the stomatal complexes, while in Vigna sinensis, they were deposited in the local cell wall thickenings of the GCs as well as at the ledges of the stomatal pore. Consideration of the presented data favors the view that in the stomatal complexes of the monocotyledon Z. mays and the dicotyledon V. sinensis, the esterified HGs contribute to the cell wall expansion taking place during GC morphogenesis and the opening of the stomatal pore. Besides, callose and the highly de-esterified HGs allow to GC cell wall regions to withstand the mechanical stresses exerted during stomatal function.