Glycyrrhizin attenuates rat ischemic spinal cord injury by suppressing inflammatory cytokines and HMGB1
Glycyrrhizin attenuates rat ischemic spinal cord injury by suppressing inflammatory cytokines and HMGB1
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DOI：
10.1038/aps.2011.151
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发表时间：
2012-01-01
影响因子：
8.2
通讯作者：
Zhou, Le-shun
中科院分区：
文献类型：
--
作者：
Gong, Gu;Yuan, Li-bang;Zhou, Le-shun
目的：研究甘草酸（Gly）对大鼠脊髓缺血损伤的神经保护作用以及核蛋白高迁移率族蛋白B1（HMGB1）在此过程中可能发挥的作用。
方法：雄性Sprague - Dawley大鼠接受45分钟的主动脉阻断以诱导短暂性腰脊髓缺血。根据改良的Tarlov评分标准评估动物的运动功能。再灌注72小时后处死动物，取出腰脊髓节段（L2 - L4）进行组织病理学检查和蛋白质印迹分析。采用酶联免疫吸附测定（ELISA）分析血清炎症细胞因子和HMGB1水平。
结果：在诱导短暂性腰脊髓缺血前30分钟静脉注射甘草酸（6mg/kg）可显著提高后肢运动功能评分，减少凋亡神经元数量，同时伴有血浆和受损脊髓中肿瘤坏死因子 - α（TNF - α）、白细胞介素 - 1β（IL - 1β）和白细胞介素 - 6（IL - 6）水平降低。此外，在再灌注期间，血清HMGB1水平与血清TNF - α、IL - 1β和IL - 6水平密切相关。
结论：研究结果表明，甘草酸可通过减少炎症细胞因子和抑制HMGB1的释放来减轻大鼠短暂性脊髓缺血损伤。
Aim: To investigate the neuroprotective effect of glycyrrhizin (Gly) against the ischemic injury of rat spinal cord and the possible role of the nuclear protein high-mobility group box 1 (HMGB1) in the process.Methods: Male Sprague-Dawley rats were subjected to 45 min aortic occlusion to induce transient lumbar spinal cord ischemia. The motor functions of the animals were assessed according to the modified Tarlov scale. The animals were sacrificed 72 h after reperfusion and the lumbar spinal cord segment (L2-L4) was taken out for histopathological examination and Western blotting analysis. Serum inflammatory cytokine and HMGB1 levels were analyzed using ELISA.Results: Gly (6 mg/kg) administered intravenously 30 min before inducing the transient lumbar spinal cord ischemia significantly improved the hind-limb motor function scores, and reduced the number of apoptotic neurons, which was accompanied by reduced levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in the plasma and injured spinal cord. Moreover, the serum HMGB1 level correlated well with the serum TNF-alpha, IL-1 beta and IL-6 levels during the time period of reperfusion.Conclusion: The results suggest that Gly can attenuate the transient spinal cord ischemic injury in rats via reducing inflammatory cytokines and inhibiting the release of HMGB1.