Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes
Identification of Basonuclin2, a DNA-binding zinc-finger protein expressed in germ tissues and skin keratinocytes
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DOI：
10.1016/j.ygeno.2003.11.009
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发表时间：
2004-05-01
期刊：
影响因子：
4.4
通讯作者：
Sinha, S
中科院分区：
文献类型：
--
作者：
Romano, RA;Li, HX;Sinha, S
我们使用生物信息学方法鉴定Basonuclin2， Basonuclin锌指转录因子家族的第二个成员。小鼠Basonuclin2蛋白由1049个氨基酸组成，在c端含有3对锌指，与Basonuclin 1具有高度的氨基酸序列相似性。此外，Basonuclin 1的其他特征结构域，如丝氨酸带和核定位信号，也存在于Basonuclin2中。我们使用基因组和计算机数据库分析来鉴定basonuclin2的人和大鼠同源物。对小鼠基因组的搜索表明，hasointclin2基因定位于4号染色体，由6个长度约为300 kb的外显子组成。Northern blot分析显示，在生殖系统组织（卵巢和睾丸）以及肾脏和皮肤中有多个basonuclin2转录本。我们证明，正如序列保守所预期的那样，重组Basonuclin2可以结合先前被认为是Basonuclin2结合位点的rRNA基因启动子中的序列。全长Basonuclin2仅定位于细胞核，表明它可能在细胞核功能中起重要作用，可能在基因调控中起重要作用。我们的研究确定Basonuclin2是Basonuclin家族的新成员。此外，与Basonuclin 1在结构和功能上的相似性表明，Basonuclin2可能在生殖细胞和皮肤角质形成细胞中发挥类似的功能。(C) 2003 Elsevier Inc.版权所有。
We used a bioinformatics approach to identify Basonuclin2, the second member of the Basonuclin zinc-finger family of transcription factors. The mouse Basonuclin2 protein consists of 1049 amino acids and contains three pairs of zinc fingers in the C-terminus that show a high level of amino acid sequence similarity with Basonuclin 1. In addition, other characteristic domains of Basonuclin 1, such as the serine strip and a nuclear localization signal, are also present in Basonuclin2. We used genomic and in silico database analysis to identify the human and rat homologs of basonuclin2. A search of the mouse genome showed that the hasointclin2 gene maps to chromosome 4 and consists of six exons spanning similar to 300 kb. Northern blot analysis revealed multiple transcripts of basonuclin2 in tissues of the reproductive system (ovary and testis) and also in kidney and skin. We demonstrate that, as expected from sequence conservation, recombinant Basonuclin2 can bind to a sequence in the promoter of a rRNA gene previously characterized as a Basonuclin-binding site. Full-length Basonuclin2 exclusively localizes to the nucleus, indicating that it likely plays an important role in nuclear function, probably in gene regulation. Our study establishes Basonuclin2 as a novel member of the Basonuclin family. Moreover, the structural and functional similarities with Basonuclin I suggest that Basonuclin2 may play an analogous function in germ cells and skin keratinocytes. (C) 2003 Elsevier Inc. All rights reserved.