Spatio-temporally expressed sorbitol transporters cooperatively regulate sorbitol accumulation in pear fruit
Spatio-temporally expressed sorbitol transporters cooperatively regulate sorbitol accumulation in pear fruit
复制标题
时空表达的山梨醇转运蛋白协同调节梨果实中山梨醇的积累
DOI：
10.1016/j.plantsci.2020.110787
复制
发表时间：
2021-02-01
期刊：
影响因子：
5.2
通讯作者：
Zhang, Shao-Ling
中科院分区：
文献类型：
--
作者：
Gu, Chao;Wu, Ruo-Fan;Zhang, Shao-Ling
山梨醇是梨种从源向库转运的主要底物。在许多山梨醇转运蛋白（SOTs）中，一些已知参与山梨醇在果实中的积累;然而，它们的具体作用尚不清楚。在这项研究中，我们研究了转录组和代谢组的各种梨样品从六个时间点，以确定这些SOTs。与先前的研究相似，叶和果实之间的山梨醇和蔗糖存在显著差异，并且在所有时间点均观察到较高浓度的山梨醇。有趣的是，我们发现山梨醇在梨果实中的积累由SOT 3，SOT 6/20，SOT 19/21和SOT 22协同介导。特别是，在1 mg L-1脱落酸和10 mg L-1吲哚乙酸处理下，果实中SOT 6/20和SOT 19/21的上调分别导致山梨醇浓度的增加。此外，山梨糖醇浓度与果糖和葡萄糖浓度呈正相关，表明山梨糖醇在确定水果甜度方面具有作用。本研究为进一步研究梨果实中糖的合成、运输、转化和积累过程奠定了基础，有助于提高果实品质。
Sorbitol is the primary substrate translocated from source to sink in pear species. Among the many sorbitol transporters (SOTs), some are known to be involved in sorbitol accumulation in fruit; however, their particular roles are unclear. In this study, we examined the transcriptome and metabolome of a variety of pear samples from six time points to identify those SOTs. Similar to previous studies, sorbitol and sucrose differed significantly between the leaf and fruit, and sorbitol was consistently observed at higher concentrations at all time points. Interestingly, we found that sorbitol accumulation in pear fruit was cooperatively mediated by SOT3, SOT6/20, SOT19/21, and SOT22. In particular, the up-regulated SOT6/20 and SOT19/21 in fruit under 1 mg L-1 abscisic acid and 10 mg L-1 indole acetic acid treatments, respectively, resulted in an increased sorbitol concentration. In addition, sorbitol concentration showed positive correlations to fructose and glucose concentrations, indicating a role for sorbitol in the determination of fruit sweetness. Together with the deduced process of sugar biosynthesis, transport, conversion, and accumulation in pear, our study provides a foundation for further research into sugar accumulation processes in pear fruit, contributing to the improvement of fruit quality.