REGULATION OF OMPC AND OMPF EXPRESSION IN ESCHERICHIA-COLI IN THE ABSENCE OF ENVZ
REGULATION OF OMPC AND OMPF EXPRESSION IN ESCHERICHIA-COLI IN THE ABSENCE OF ENVZ
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DOI：
10.1128/jb.170.11.5080-5085.1988
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发表时间：
1988-11-01
影响因子：
3.2
通讯作者：
INOUYE, M
中科院分区：
文献类型：
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作者：
FORST, S;DELGADO, J;INOUYE, M
编码主要外膜蛋白OmpF和OMPC的基因在大肠杆菌中的表达受ompR和envZ的调控，ompR编码转录激活蛋白OmpR，envZ编码位于内膜的受体样蛋白。为了研究EnvZ在渗透调节的孔蛋白基因表达中的作用，我们分析了缺乏envZ功能的细胞中OmpF和OMPC的产生。我们发现，EnzZ是在最低限度的培养液中生长的细胞最大限度地产生OMPC所必需的，但对于向高渗透压介质转变过程中发生的OMPC的有效诱导并不是必需的。相反，在缺乏envZ功能的细胞中，OmpF的产生与亲本菌株相似，而在没有EnvZ的情况下，在向高渗透压介质转变的过程中，OmpF的抑制是不完整的。这些结果在EnvZ在OmpF和OMPC表达中的假定作用的背景下进行了讨论。
The expression of the genes encoding the major outer membrane porin proteins OmpF and OmpC in Escherichia coli is regulated by ompR, which encodes the transcriptional activator protein OmpR, and envZ, which encodes a receptorlike protein located in the inner membrane. To examine the role of EnvZ in the expression of the osmoregulated porin genes, we analyzed the production of OmpF and OmpC in cells that lack envZ function. We show that EnzZ is required for the maximal production of OmpC in cells grown in minimal medium but is not essential for the efficient induction of OmpC that occurs during a shift to a high-osmolarity medium. In contrast, the production of OmpF in cells that lack envZ function was similar to that of the parent strain, whereas OmpF repression during a shift to a high-osmolarity medium was incomplete in the absence of EnvZ. These results are discussed in the context of the putative role of EnvZ in the expression of ompF and ompC.