Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla
Simultaneous monitoring of acetylcholine and catecholamine release in the in vivo rat adrenal medulla
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DOI：
10.1016/j.neuint.2003.09.001
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发表时间：
2004-06-01
影响因子：
4.2
通讯作者：
Sunagawa, K
中科院分区：
文献类型：
--
作者：
Akiyama, T;Yamazaki, T;Sunagawa, K
为了在体内状态下同时监测节前肾上腺交感神经末梢的乙酰胆碱释放和节后肾上腺嗜铬细胞的儿茶酚胺释放，我们对麻醉大鼠应用微透析技术。将透析探针植入左肾上腺髓质并灌注含有新斯的明（胆碱酯酶抑制剂）的林格氏溶液。横断内脏神经后，我们电刺激内脏神经或通过透析探针局部给予乙酰胆碱2分钟，并研究透析液乙酰胆碱、胆碱、去甲肾上腺素和肾上腺素的反应。神经刺激前透析液中未检测到乙酰胆碱，但神经刺激后检测到大量乙酰胆碱。相反，在刺激前在透析液中检测到胆碱，并且透析液胆碱浓度不随重复神经刺激而变化。外源性乙酰胆碱（10μM）和神经刺激（2Hz）之间估计的间质乙酰胆碱水平和透析液儿茶酚胺反应几乎相同。透析液乙酰胆碱、去甲肾上腺素和肾上腺素反应与电神经刺激的频率相关，透析液去甲肾上腺素和肾上腺素反应与透析液乙酰胆碱反应定量相关。六甲铵（一种烟碱受体拮抗剂）和阿托品（一种毒蕈碱受体拮抗剂）都不影响透析液乙酰胆碱对神经刺激的反应。微透析技术使得在体内状态下同时评估节前肾上腺交感神经和节后肾上腺嗜铬细胞的活动成为可能，并提供肾上腺髓质输入输出关系的定量信息。 (C) 2003 Elsevier Ltd. 保留所有权利。
To simultaneously monitor acetylcholine release from pre-ganglionic adrenal sympathetic nerve endings and catecholamine release from post-ganglionic adrenal chromaffin cells in the in vivo state, we applied microdialysis technique to anesthetized rats. Dialysis probe was implanted in the left adrenal medulla and perfused with Ringer's solution containing neostigmine (a cholinesterase inhibitor). After transection of splanchnic nerves, we electrically stimulated splanchnic nerves or locally administered acetylcholine through dialysis probes for 2 min and investigated dialysate acetylcholine, choline, norepinephrine and epinephrine responses. Acetylcholine was not detected in dialysate before nerve stimulation, but substantial acetylcholine was detected by nerve stimulation. In contrast, choline was detected in dialysate before stimulation, and dialysate choline concentration did not change with repetitive nerve stimulation. The estimated interstitial acetylcholine levels and dialysate catecholamine responses were almost identical between exogenous acetylcholine (10 muM) and nerve stimulation (2 Hz). Dialysate acetylcholine, norepinephrine and epinephrine responses were correlated with the frequencies of electrical nerve stimulation, and dialysate norepinephrine and epinephrine responses were quantitatively correlated with dialysate acetylcholine responses. Neither hexamethonium (a nicotinic receptor antagonist) nor atropine (a muscarinic receptor antagonist) affected the dialysate acetylcholine response to nerve stimulation. Microdialysis technique made it possible to simultaneously assess activities of pre-ganglionic adrenal sympathetic nerves and post-ganglionic adrenal chromaffin cells in the in vivo state and provided quantitative information about input-output relationship in the adrenal medulla. (C) 2003 Elsevier Ltd. All rights reserved.