Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA
Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA
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DOI：
10.1016/s1097-2765(00)80163-x
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发表时间：
1998-11-01
期刊：
影响因子：
16
通讯作者：
Green, MR
中科院分区：
文献类型：
--
作者：
Apone, LM;Virbasius, CA;Green, MR
RNA聚合酶II通用转录因子TFIID是包含TATA盒结合蛋白（TBP）和相关因子（TAF（II））的多亚基复合物。酵母实验表明，虽然大多数TAF（II）是生存所必需的，但许多基因在单个甚至多个yTAF（II）失活后正常转录。在这里，我们分析yTAF（II）17，最近发现存在于佐贺HAT复合物以及TFIID。通过温度敏感性突变或缺失使yTAF（II）17功能失活会导致许多（但不是全部）基因的转录丢失。上游激活序列（UAS）包含激活剂结合位点，是使基因yTAF（II）17依赖的区域。结合以前的研究，我们的研究结果表明，不同的TAF（II）有显着不同的属性。
The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA box-binding protein (TBP) and associated factors (TAF(II)s). Experiments in yeast have shown that although most TAF(II)s are required for viability, many genes are transcribed normally upon inactivation of individual and even multiple yTAF(II)s. Here we analyze yTAF(II)17, recently found to be present in both the SAGA HAT complex as well as TFIID. Functional inactivation of yTAF(II)17 by temperature-sensitive mutation or depletion results in loss of transcription of many, but not all, genes. The upstream activating sequence (UAS), which contains the activator binding sites, is the region that renders a gene yTAF(II)17 dependent. In conjunction with previous studies, our results reveal that different TAF(II)s have remarkably distinct properties.