Modeling of oxygen transport across tumor multicellular layers
Modeling of oxygen transport across tumor multicellular layers
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DOI：
10.1016/j.mvr.2006.11.001
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Beatty, Alexis L.
中科院分区：
文献类型：
--
作者：
Braun, Rod D.;Beatty, Alexis L.
目的：肿瘤氧水平在肿瘤对不同治疗的反应中起主要作用。本研究的目的是开发一种方法来确定氧的运输特性，在最近开发的三维模型的肿瘤实质，多细胞层（MCL）。方法：OCM-1人脉络膜黑色素瘤细胞生长为三维MCLs胶原包被的培养板插入。使用凹式阴极氧微电极测量从自由表面到插入膜的8个不同MCL的氧张力（PO 2）分布。用4种不同的一维扩散模型拟合PO 2浓度分布：1区模型、2区模型和3区模型，每个区的耗氧量（q）相同，而改进的3区模型中有一个中心区，q=0，PO 2 = 0。结果：根据中心区缺氧的情况，两区模型和改进的3区模型拟合的PO 2浓度分布最好。插入膜附近的肿瘤细胞的消耗高于接近自由表面的细胞（分别为33.1 +/- 13.6 x 10（-4）和11.8 +/- 6.7 x 10（-4）mm Hg/mu m（2））。结论：该模型可用于确定MCL中的氧合和消耗，特别是对于不能生长为球状体的细胞系。该模型将为体外肿瘤氧合重要参数的研究奠定基础。(c)2006爱思唯尔公司All rights reserved.
Purpose: Tumor oxygen level plays a major role in the response of tumors to different treatments. The purpose of this study was to develop a method of determining oxygen transport properties in a recently developed 3D model of tumor parenchyma, the multicellular layer (MCL).Methods: OCM-1 human choroidal melanoma cells were grown as 3D MCLs on collagen-coated culture plate inserts. A recessed-cathode oxygen microelectrode was used to measure oxygen tension (PO2) profiles across 8 different MCL from the free surface to the insert membrane. The profiles were fitted to four different one-dimensional diffusion models: 1-, 2-, and 3-region models with uniform oxygen consumption (q) in each region and a modified 3-region model with a central region where q=0 and PO2=0.Results: Depending upon the presence of a central region of anoxia, the PO2 profiles were fitted best by either the two-region model or the modified 3-region model. Consumption of tumor cells near the insert membrane was higher than that of cells close to the free surface (33.1 +/- 13.6 x 10(-4) vs. 11.8 +/- 6.7 x 10(-4) mm Hg/mu m(2), respectively).Conclusions: The model is useful for determining oxygenation and consumption in MCL, especially for cell lines that cannot be grown as spheroids. In the future, this model will pen-nit the study of parameters important in tumor oxygenation in vitro. (c) 2006 Elsevier Inc. All rights reserved.