Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus
Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus
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DOI：
10.1111/j.1600-0714.2011.01084.x
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Nylander, K.
中科院分区：
文献类型：
--
作者：
Danielsson, K.;Wahlin, Y. B.;Nylander, K.
背景：口腔扁平苔藓(OLP)是一种病因不明的慢性口腔黏膜炎症性疾病，约占人口总数的2%。MicroRNAs是一种小的非编码RNA，参与人类疾病的发育、分化和进展等正常过程。本研究的目的是研究口腔扁平苔藓中miR-21、miR-125b和miR-203的表达，并比较它们的潜在靶点抑癌基因p53及其相关的p63的RNA水平。方法：在20例口腔扁平苔藓患者和20名年龄和性别匹配的健康对照组的活检组织中，激光切割并分析miR-21、miR-125b、miR-203、p53和p63的表达。与正常口腔黏膜相比，口腔扁平苔藓组织中P53和Delta Np63RNA表达下调。DNp63与miR-203、miR-21与p53之间存在显著负相关。结论：研究结果提示所研究的microRNA在口腔扁平苔藓中所起的作用。口腔病理医学杂志(2012)41：9095
BACKGROUND: Oral lichen planus (OLP), which is a chronic inflammatory disease of the oral mucosa with unknown etiology, affects about 2% of the population. MicroRNAs are small non-coding RNAs involved in normal processes such as development and differentiation as well as progression of human diseases. The aim of this study was to investigate the expression of miR-21, miR-125b, and miR-203 and to compare RNA levels of their potential targets, the tumor suppressor p53 and its relative p63, both known to be deregulated in OLP.METHODS: In biopsies from 20 patients with OLP and 20 age- and sex-matched healthy controls, epithelium was laser dissected and analyzed for the expression of miR-21, miR-125b, miR-203, p53, and p63 using qRT/PCR.RESULTS: Increased expression of miR-21 and miR-203, decreased expression of miR-125, and down-regulation of p53 and Delta Np63 RNA were seen in OLP compared to normal oral mucosa. When comparing microRNA expression to levels of p53 and p63 RNA, a significant negative correlation was seen between DNp63 and miR-203 and between miR-21 and p53, respectively.CONCLUSION: Results indicate a role for the studied microRNAs in changes seen in OLP. J Oral Pathol Med (2012) 41: 9095