GzSNF1 Is Required for Normal Sexual and Asexual Development in the Ascomycete Gibberella zeae
GzSNF1 Is Required for Normal Sexual and Asexual Development in the Ascomycete Gibberella zeae
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DOI：
10.1128/ec.00176-08
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Lee, Yin-Won
中科院分区：
文献类型：
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作者：
Lee, Seung-Ho;Lee, Jungkwan;Lee, Yin-Won
酵母的蔗糖非发酵1（SNF1）蛋白激酶在葡萄糖饥饿时葡萄糖抑制基因的转录中起着重要作用。在这项研究中，我们删除了直向同源物的SNF1从赤霉菌的功能进行了表征，通过使用基因置换策略。在不同的碳源上，缺失突变体（Delta GzSNF1）的菌丝生长减少了21%至74%。Delta GzSNF1突变体对大麦的毒力下降，细胞壁降解酶基因的表达减少。最明显的表型变化是在有性和无性发育。三角洲GzSNF1突变体产生少30%的子囊壳，成熟更慢，子囊含有1至8个形状异常的子囊孢子。其中仅GzSNF1催化结构域缺失的突变体具有与Delta GzSNF1菌株相同的表型变化，但在具有调控结构域缺失的突变体中表型不那么极端。在三角洲GzSNF1突变体之间的异交中，每个子囊壳包含类似于70%的异常子囊孢子，并且类似于50%的子囊在测试的单个位点中显示出意想不到的分离模式。Δ GzSNF1突变体的无性孢子比野生型菌株的孢子短，隔膜少。与野生型菌株相比，Delta GzSNF1突变体的子囊孢子和分生孢子的萌发和成核延迟。GzSNF1的表达和定位依赖于真菌的发育阶段。这些结果表明，GzSNF1是至关重要的正常性和无性发育，除了毒力和利用替代碳源。
The sucrose nonfermenting 1 (SNF1) protein kinase of yeast plays a central role in the transcription of glucose-repressible genes in response to glucose starvation. In this study, we deleted an ortholog of SNF1 from Gibberella zeae to characterize its functions by using a gene replacement strategy. The mycelial growth of deletion mutants (Delta GzSNF1) was reduced by 21 to 74% on diverse carbon sources. The virulence of Delta GzSNF1 mutants on barley decreased, and the expression of genes encoding cell-wall-degrading enzymes was reduced. The most distinct phenotypic changes were in sexual and asexual development. Delta GzSNF1 mutants produced 30% fewer perithecia, which matured more slowly, and asci that contained one to eight abnormally shaped ascospores. Mutants in which only the GzSNF1 catalytic domain was deleted had the same phenotype changes as the Delta GzSNF1 strains, but the phenotype was less extreme in the mutants with the regulatory domain deleted. In outcrosses between the Delta GzSNF1 mutants, each perithecium contained similar to 70% of the abnormal ascospores, and similar to 50% of the asci showed unexpected segregation patterns in a single locus tested. The asexual spores of the Delta GzSNF1 mutants were shorter and had fewer septa than those of the wild-type strain. The germination and nucleation of both ascospores and conidia were delayed in Delta GzSNF1 mutants in comparison with those of the wild-type strain. GzSNF1 expression and localization depended on the developmental stage of the fungus. These results suggest that GzSNF1 is critical for normal sexual and asexual development in addition to virulence and the utilization of alternative carbon sources.