Enterolignan-producing phenotypes are associated with increased gut microbial diversity and altered composition in premenopausal women in the United States.
Enterolignan-producing phenotypes are associated with increased gut microbial diversity and altered composition in premenopausal women in the United States.
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DOI：
10.1158/1055-9965.epi-14-0262
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Lampe JW
中科院分区：
文献类型：
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作者：
Hullar MA;Lancaster SM;Li F;Tseng E;Beer K;Atkinson C;Wähälä K;Copeland WK;Randolph TW;Newton KM;Lampe JW
植物性食品中的木脂素由肠道细菌代谢成肠木脂素、肠二醇组分和肠内酯组分。肠脂素具有重要的生物活性，对预防癌症和慢性病具有重要作用。我们研究了肠道微生物群落(GMC)的组成，作为人类肠道木脂素暴露的一个贡献者。我们从115名绝经前(40-45岁)美国妇女的3天饮食记录中评估了粪便中的GMC、尿肠木脂素排泄量和饮食之间的关系。用GC/MS测定尿肠木脂素，用16S rRNA基因454焦磷酸测序评价GMC。序列在Silva(www.arb-silva.de)中进行比对。操作分类单位(OTU)的序列相似性为97%。进行分类学分类，评估与ENL产生相关的α和β多样性。采用多变量分析和回归分析建立肠木脂素排泄与GMC之间的关系模型。用单变量分析和岭回归分析鉴定与ENL产生相关的细菌。在调整膳食纤维摄入量和肥胖后，我们发现ENL排泄量与GMC(p=0.0007)或GMC的多样性(p=0.01)呈显著正相关。与ENL产量高相关的GMC是明显的(unirac，p<；0.003，mrpp)，并富含Moryella spp.、Acetanacteria spp.、Fastidiosipila spp.和Strehop bacillus spp.GMC的多样性和组成与人类接触肠脂素的增加有关。肠道微生物多样性和组成的差异解释了影响环境暴露和影响人类健康的肠道代谢过程的差异。
Lignans in plant foods are metabolized by gut bacteria to the enterolignans, enterodiol (END) and enterolactone (ENL). Enterolignans have biologic activities important to the prevention of cancer and chronic diseases. We examined the composition of the gut microbial community (GMC) as a contributor to human enterolignan exposure. We evaluated the association between the GMC in stool, urinary enterolignan excretion, and diet from a 3-day food record in 115 premenopausal (ages 40–45 y) women in the United States. Urinary enterolignans were measured using GC/MS. The GMC was evaluated using 454 pyrosequencing of the 16S rRNA gene. Sequences were aligned in SILVA (www.arb-silva.de). Operational taxonomic units (OTU) were identified at 97% sequence similarity. Taxonomic classification was performed and alpha and beta diversity in relationship to ENL production were assessed. Multivariate analysis and regression were used to model the association between enterolignan excretion and the GMC. Bacteria associated with ENL production were identified using univariate analysis and ridge regression. After adjusting for dietary fiber intake and adiposity, we found a significant positive association between ENL excretion and either the GMC (p=0.0007), or the diversity of the GMC (p=0.01). The GMC associated with high ENL production was distinct (UNIFRAC, p<0.003, MRPP) and enriched in Moryella spp., Acetanaerobacterium spp., Fastidiosipila spp., and Streptobacillus spp. Diversity and composition of the GMC are associated with increased human exposure to enterolignans. Differences in gut microbial diversity and composition explain variation in gut metabolic processes that impact environmental exposures and influences human health.