Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultured cells from alpine Chorispora bungeana
Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultured cells from alpine Chorispora bungeana
复制标题
几种抗氧化酶与高山白僵菌悬浮培养细胞抗快速冷冻能力的关系
DOI：
10.1016/j.cryobiol.2005.12.001
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发表时间：
2006-04-01
期刊：
影响因子：
2.7
通讯作者：
An, LZ
中科院分区：
文献类型：
--
作者：
Guo, FX;Zhang, MX;An, LZ
高山离子芥（十字花科）是一种能在暴风雪中存活的罕见高山冰雪带植物。在本文中，我们试图探究自氧化速率（AR）以及与植物细胞抗冻机制相关的抗氧化酶可能起到的作用。结果表明，当在25℃下生长的悬浮培养物突然暴露于-8℃下15天，在9天内氯化三苯基四氮唑还原未受影响，并且与对照相比，AR保持在较低水平。这表明在整个冷冻试验过程中，细胞维持了大量的可溶性蛋白质，细胞膜的完整性也未受破坏。此外，平均而言，超氧化物歧化酶、脱氢抗坏血酸还原酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶等抗氧化酶的活性在受冷冻胁迫的细胞中显著增强。过氧化物酶活性在冷冻后不久显著增加，可能是为了弥补细胞中过氧化氢酶活性早期的下降。统计分析表明，在从25℃暴露于-8℃后，培养细胞对快速冷冻的抗性与抗氧化酶活性之间呈负相关，这表明冷冻导致的细胞活力降低激活了一系列抗氧化酶，从而使细胞保持完整。所有这些发现都表明这些抗氧化酶之间存在协同作用，导致较低的自氧化速率，这有助于保护细胞膜，并在高山离子芥悬浮培养细胞对突然冷冻的抗性中发挥重要作用。（c）2005爱思唯尔公司。保留所有权利。
Chorispora bungeana Fisch. & C.A. Mey (Crucifer) is a rare alpine subnival plant surviving sudden snowstorms. In this paper, we have attempted to explore possible roles of autoxidation rate (AR) and the antioxidant enzymes associated with cryoprotective mechanisms in the plant cells. The results showed that when the suspension cultures growing at 25 degrees C were suddenly exposed to -8 degrees C for 15 days, 2, 3,5-triphenyltetrazolium chloride reduction was not affected within 9 days and AR remained at a low level in comparison with controls. This indicated that the cells maintained considerable amounts of soluble protein and the integrity of the cell membranes was intact during the whole freezing test. Furthermore, oil average, the activity of antioxidant enzymes Such as Superoxide dismutase, dehydroascorbate reductase, ascorbate peroxidase and glutathione reductase were prominently enhanced in the freezing-stressed cells. Peroxidase activity significantly increased soon after freezing, possibly to make Lip for the early decrease of catalase activity in the cells. Statistical analysis showed negative correlations between resistance to rapid freezing and antioxidant enzyme activity in the cultured cells after exposure from 25 to -8 degrees C, indicating that the reduction of cell viability with freezing activates a combination of antioxidant enzymes that results in intact cells. All of these findings Suggest a synergy between these antioxidant enzymes, leading to a low autoxidation rate that contributes to the protection of the cell membranes and plays all important role ill the resistance of suspension Cultured cells of C bungeana to sudden freezing. (c) 2005 Elsevier Inc. All rights reserved.