A colorimetric immunoassay based on glucose oxidase-induced AuNP aggregation for the detection of fumonisin B1
A colorimetric immunoassay based on glucose oxidase-induced AuNP aggregation for the detection of fumonisin B1
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基于葡萄糖氧化酶诱导的 AuNP 聚集的比色免疫分析法检测伏马菌素 B-1
DOI：
10.1016/j.talanta.2018.04.018
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发表时间：
2018-08-15
期刊：
影响因子：
6.1
通讯作者：
Xiong, Yonghua
中科院分区：
文献类型：
--
作者：
Chen, Xirui;Liang, Yi;Xiong, Yonghua
裸眼读出比色信号为基础的分析是有希望的高通量筛选检测和点护理诊断在资源有限的地区。本文开发了一种基于金纳米颗粒（AuNP）聚集的新型直接竞争性等离子体酶联免疫吸附试验（dc-pELISA），具有高灵敏度和强大的裸眼读取信号，并用于检测伏马菌素B-1 （FB1）。通过辣根过氧化物酶(HRP)/过氧化氢(H2O2)/酪胺（TYR）体系诱导AuNP聚集，导致颜色由红色变为蓝色。在该体系中，H2O2通过gox介导的葡萄糖氧化反应产生，FB1-GOx作为竞争抗原。提出的pELISA证明了对FB1在3.125 ng mL（-1）至25 ng mL（-1）范围内的良好线性检测，颜色从深蓝到红色变化生动，肉眼观察到的截止限为12.5 ng mL（-1）。加样回收率为76.5% ~ 96.8%，相对标准偏差为4.88，相对标准偏差为16.4%。同时，该方法与传统的ELISA法在盲检FBI玉米样品中具有很好的一致性（R-2 = 0.927）。此外，该方法对FB1阳性玉米样品的检测结果与超高效液相色谱法的结果也具有较高的一致性。这些结果表明，所提出的比色ELISA定量检测实际玉米样品中FB1的准确性和精密度可接受。
Naked-eye readout colorimetric signal-based assays are promising for high-throughput screening detection and point-of-care diagnostics in resource-constrained regions. Here, a novel direct competitive plasmonic enzyme linked immunosorbent assay (dc-pELISA) based on gold nanoparticle (AuNP) aggregation with highly sensitive and robust naked-eye readout signal was developed and used to detect fumonisin B-1 (FB1). AuNP aggregation was induced by a horseradish peroxidase (HRP)/hydrogen peroxide (H2O2)/tyramine (TYR) system, resulting in a dramatic color change from red to blue. In this system, H2O2 was produced via GOx-mediated glucose oxidation reaction, and FB1-GOx was used as a competitive antigen. The proposed pELISA demonstrated good linear detection of FB1 from 3.125 ng mL(-1) to 25 ng mL(-1) with a vivid color change from deep blue to red and a cutoff limit of 12.5 ng mL(-1) observed by the naked eye. The average recoveries for FB1-spiked corn samples ranged from 76.5% to 96.8% with a relative standard derivation of 4.88 similar to 16.4%. Meanwhile, the proposed method exhibited excellent agreement (R-2 = 0.927) with the conventional ELISA method in blindly detecting FBI spiked corn samples. Additionally, the results of the proposed method for FB1 positive corn samples also showed a high consistency with those of the ultra performance liquid chromatography method. These results indicated acceptable accuracy and precision of the proposed colorimetric ELISA for quantitative detection of FB1 in actual corn samples.