Maternal aggression in endothelial nitric oxide synthase-deficient mice
Maternal aggression in endothelial nitric oxide synthase-deficient mice
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DOI：
10.1006/hbeh.2000.1595
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发表时间：
2000-08-01
影响因子：
3.5
通讯作者：
Nelson, RJ
中科院分区：
文献类型：
--
作者：
Gammie, SC;Huang, PL;Nelson, RJ
哺乳期的雌性啮齿动物通过表达对入侵者的猛烈攻击来保护它们的幼崽，这种攻击被称为母性攻击。缺乏神经元型一氧化氮合酶(nNOS-/-)基因的小鼠表现出明显的母体攻击行为受损，但男性攻击行为增加，这表明nNOS产生的一氧化氮(NO)在母体攻击和男性攻击中具有相反的作用。相比之下，缺乏内皮型一氧化氮合酶基因(eNOS-/-)的小鼠几乎没有表现出男性攻击行为，这表明eNOS产生的NO促进了男性攻击行为。在本研究中，eNOS-/-小鼠的母体攻击行为与野生型(WT)小鼠相比是正常的，表现出攻击性的百分比、对雄性入侵者的平均攻击次数以及攻击雄性入侵者的总时间都是正常的。ENOS-/-雌性也表现出正常的幼崽取回行为。由于催乳期WT小鼠下丘脑神经元中的瓜氨酸显著升高，这是一种NO合成的间接标记物，与母亲的攻击行为有关，因此我们检查了eNOS-/雌性小鼠在一次攻击性遭遇后大脑中的瓜氨酸免疫反应。攻击性eNOS-/-雌鼠下丘脑视前内侧核和室旁区的瓜氨酸水平显著高于未受刺激的哺乳期eNOS-/-雌鼠。综上所述，这些结果表明，eNOS产生的NO既不促进也不抑制母亲的攻击，并且eNOS产生的NO在母体和男性的攻击中具有不同的作用。(C)2000本学术出版社。
Lactating female rodents protect their pups by expressing fierce aggression, termed maternal aggression, toward intruders. Mice lacking the neuronal nitric oxide synthase gene (nNOS-/-) exhibit significantly impaired maternal aggression, but increased male aggression, suggesting that nitric oxide (NO) produced by nNOS has opposite actions in maternal and male aggression. In contrast, mice lacking the endothelial nitric oxide synthase gene (eNOS-/-) exhibit almost no male aggression, suggesting that NO produced by eNOS facilitates male aggression. In the present study, maternal aggression in eNOS-/- mice was examined and found to be normal relative to wild-type (WT) mice in terms of the percentage displaying aggression, the average number of attacks against a male intruder, and the total amount of time spent attacking the male intruder. The eNOS-/-females also displayed normal pup retrieval behavior. Because a significant elevation of citrulline, an indirect marker of NO synthesis, occurs in neurons of the hypothalamus of lactating WT mice in association with maternal aggression, we examined the brains of eNOS-/females for citrulline immunoreactivity following an aggressive encounter. The aggressive eNOS-/- females exhibited a significant elevation of citrulline in the medial preoptic nucleus and the subparaventricular zone of the hypothalamus relative to unstimulated lactating eNOS-/- females. Taken together, these results suggest that NO produced by eNOS neither facilitates nor inhibits maternal aggression and that NO produced by eNOS has a different role in maternal and male aggression. (C) 2000 Academic press.