The presence of oleate stabilized ZnO nanoparticles (NPs) and reduced the toxicity of aged NPs to Caco-2 and HepG2 cells
The presence of oleate stabilized ZnO nanoparticles (NPs) and reduced the toxicity of aged NPs to Caco-2 and HepG2 cells
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DOI：
10.1016/j.cbi.2017.10.002
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发表时间：
2017-12-25
影响因子：
5.1
通讯作者：
Cao, Yi
中科院分区：
文献类型：
--
作者：
Fang, Xin;Jiang, Leying;Cao, Yi
食品成分的存在可能会改变纳米颗粒（NPs）的胶体性质和毒性。在这项研究中，ZnO纳米颗粒对Caco-2和HepG 2细胞的毒性进行了评估，重点是ZnO纳米颗粒和油酸（OA）之间的相互作用。OA的存在增加了ZnO纳米粒子的紫外-可见光谱和流体力学尺寸，降低了Zeta电位，并显着减少了Zn离子从ZnO纳米粒子溶解中的释放，这表明OA可以包覆ZnO纳米粒子并稳定ZnO纳米粒子。暴露于ZnO纳米颗粒显著诱导Caco-2和HepG 2细胞的细胞毒性，与细胞内Zn离子的增加有关，但与超氧化物无关。当OA加入到新鲜制备的ZnO纳米粒悬浮液中时，ZnO纳米粒诱导的细胞毒性、细胞内锌离子和超氧化物歧化酶没有显著影响。然而，当ZnO纳米粒子老化24小时与OA的存在下，ZnO纳米粒子的细胞毒性Caco-2和HepG 2细胞显着降低，与细胞内锌离子的减少。本研究的结果表明，OA的存在可以增加ZnO纳米颗粒的胶体稳定性，从而降低老化后ZnO纳米颗粒的毒性，这与细胞内Zn离子的积累减少有关。
The presence of food components may alter the colloidal aspects and toxicity of nanoparticles (NPs). In this study, the toxicity of ZnO NPs to Caco-2 and HepG2 cells was assessed, with the emphasis on the interactions between ZnO NPs and oleate (OA). The presence of OA increased UV-Vis spectra and hydrodynamic sizes, decreased Zeta potential, and markedly reduced the release of Zn ions from the dissolution of ZnO NPs, which combined indicated that OA could coat ZnO NPs and stabilize ZnO NPs. Exposure to ZnO NPs significantly induced cytotoxicity to Caco-2 and HepG2 cells, associated with increased intracellular Zn ions but not superoxide. When OA was added to the freshly prepared ZnO NP suspensions, the cytotoxicity, intracellular Zn ions and superoxide induced by ZnO NPs were not significantly affected. However, when ZnO NPs were aged for 24 h with the presence of OA, the cytotoxicity of ZnO NPs to Caco-2 and HepG2 cells was significantly reduced, associated with a reduction of intracellular Zn ions. The results from this study suggested that the presence of OA could increase colloidal stability of ZnO NPs and consequently reduce the toxicity of ZnO NPs after aging associated with reduced accumulation of intracellular Zn ions.