Burkholderia heleia sp nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam
Burkholderia heleia sp nov., a nitrogen-fixing bacterium isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of Vietnam
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DOI：
10.1099/ijs.0.015198-0
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发表时间：
2010-05-01
影响因子：
2.8
通讯作者：
Sunairi, Michio
中科院分区：
文献类型：
--
作者：
Aizawa, Tomoko;Nguyen Bao Ve;Sunairi, Michio
从生长在越南酸性硫酸盐土壤区高酸性沼泽（pH 2-4）中的水生植物--甜荸荠（Eleocharis dulcis）中分离到3株固氮菌，分别命名为SA 41（T）、SA 42和SA 53。分离物为革兰氏阴性、需氧、不形成孢子的杆状细菌，细胞宽度为0.6-0.7 μ m，长度为1.5-1.7 μ m。它们在pH 3.0和7.0之间以及在17和37摄氏度之间表现出良好的生长。生物体含有泛醌Q-8作为主要的类异戊二烯醌，和C-16：0、C-17：0环、C-18：1 ω 7 c和总和特征3（C-16：1 ω 7 c和/或异-C-15：0 2-OH）作为主要脂肪酸。它们的脂肪酸谱与其他伯克霍尔德菌属物种的报告相似。这些菌株的DNA G+C含量为64mol%。根据16 S rRNA基因序列的相似性，这些菌株被证明属于伯克霍尔德氏菌属。虽然它们与大西洋伯克霍尔德菌、含羞草伯克霍尔德菌、铁伯克霍尔德菌和热带伯克霍尔德菌的16 S rRNA基因序列相似性分别为98.5%、98.2%、98.0%和97.0%，但在系统发育树中形成了一个明显的类群，菌株SA 41（T）与这些种的DNA-DNA相关性分别为39%、41%、39%和33%。分别生理和生化试验的结果，包括全细胞蛋白质模式分析，允许表型分化的这些菌株从公布的伯克霍尔德菌属物种。因此，菌株SA 41（T）、SA 42和SA 53代表了一个新的种，建议将其命名为Burkholderia heleia sp. nov.。模式菌株为SA 41（T）（=NBRC 101817（T）= VTCC-D 6 -7（T））。
Nitrogen-fixing bacteria, strains SA41(T), SA42 and SA53, were isolated from an aquatic plant, Eleocharis dulcis, that grows in highly acidic swamps (pH 2-4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6-0.7 mu m and a length of 1.5-1.7 mu m. They showed good growth between pH 3.0 and 7.0, and between 17 and 37 degrees C. The organisms contained ubiquinone Q-8 as the predominant isoprenoid quinone, and C-16:0, C-17:0 cyclo, C-18:1 omega 7c and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH) as major fatty acids. Their fatty acid profiles are similar to those reported for other Burkholderia species. The DNA G+C content of these strains was 64 mol%. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Burkholderia. Although their calculated 16S rRNA gene sequence similarity values to Burkholderia silvatlantica, Burkholderia mimosarum, Burkholderia ferrariae and Burkholderia tropica were 98.5, 98.2, 98.0 and 97.0%, respectively, the isolates formed a distinct group in phylogenetic trees, and the DNA-DNA relatedness values of strain SA41(T) to these species were 39, 41, 39 and 33%, respectively. The results of physiological and biochemical tests, including whole-cell protein pattern analysis, allowed phenotypic differentiation of these strains from the published Burkholderia species. Therefore, strains SA41(T), SA42 and SA53 represent a novel species for which the name Burkholderia heleia sp. nov. is proposed. The type strain is SA41(T) (=NBRC 101817(T)=VTCC-D6-7(T)).