The role of pH in the isolation of nerve ending particles which transport GABA and glutamic acid
The role of pH in the isolation of nerve ending particles which transport GABA and glutamic acid
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pH 在分离运输 GABA 和谷氨酸的神经末梢颗粒中的作用
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
R. Hitzemann
中科院分区：
文献类型：
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作者：
R. Hitzemann
最近，我们研究了CABA转运到大鼠脑神经末梢颗粒中的各种结构、地形和发育方面（HITZEMANN &洛，1978 a，B，c）。在这些研究过程中，我们观察到，通过标记的GABA转运速率测量的活神经末梢的产量在不同制备物之间变化显著。为了找到这些变异的原因，我们开始对用于制备神经末梢的技术进行系统的研究。我们的初步结果表明，在各种分离程序中使用的蔗糖溶液的pH值是确定可行的神经末梢产量的最关键因素之一。因此，我们进行了详细的调查的作用pH值在准备神经末梢，用于测量标记的GABA运输。我们的研究结果，如本报告所述，表明7.8的pH值是制备这种神经末梢的最佳条件。
RECENTLY we have examined various structural, topographical and developmental aspects of CABA transport into rat brain nerve ending particles (HITZEMANN & LOH, 1978a, b, c), During the course of these studies, we observed that the yield of viable nerve endings, as measured by the rate of labeled GABA transport, varied significantly from preparation to preparation. In an attempt to locate the cause or causes of these variations, we began a systematic investigation of the technique used to prepare the nerve endings. Our initial results indicated that the pH of the sucrose solutions used in the various isolation procedures was one of the single most critical factors in determining the viable nerve ending yield. We, therefore, undertook a detailed investigation of the role p H plays in preparing nerve endings that are used to measure labeled GABA transport. Our results, as described in this report, suggest that a pH of 7.8 is optimum for preparing such nerve endings.