Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb
Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb
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DOI：
10.1369/jhc.5a6669.2005
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发表时间：
2005-12-01
影响因子：
3.2
通讯作者：
Sasano, Y
中科院分区：
文献类型：
--
作者：
Nakamura, M;Sone, S;Sasano, Y
细胞外基质(ECM)重塑是通过骨发育过程中ECM分子的产生和降解来实现的。ADAMTS(一种带有凝血酶敏感蛋白1基序的去整合素和金属蛋白酶)构成了一个胞外蛋白家族，与切割该蛋白有关。本研究采用RT-PCR和原位杂交技术，研究了Verscan和ADAMTS1、4、5基因在大鼠下颌骨和后肢骨发育过程中的表达。用免疫组织化学方法对Verscan进行定位。从出生后第14天到出生后6周，骨的发育过程分为成骨开始阶段、编织骨阶段和板层骨阶段。Verscan蛋白在编织骨基质中含量丰富，但在板层骨基质中含量较少。Versican基因在一些成骨细胞中显著表达，并有相应的同源蛋白定位。ADAMTS1、ADAMT4和ADAMT5在时间和空间上的表达模式与Versican相似。这些结果表明，在下颌骨和后肢的骨发育过程中，富含凡西康的编织骨转变为含有少量凡西康的板层骨，其中一些成骨细胞可能通过分泌ADAMTS1、4和5参与了凡西康的生产和降解。
Extracellular matrix (ECM) remodeling is achieved by both production and degradation of ECM molecules during bone development. ADAMTS (a disintegrin and metalloprotease with thrombospondin type 1 motifs) constitutes a family of extracellular proteases which are implicated in cleaving the protein versican. The present study was designed to investigate the expression of versican and ADAMTS1, 4, and 5 mRNA during bone development in rat mandibles and hind limbs by RT-PCR and in situ hybridization. Versican was localized by immunohistochemistry. The process of bone development from day 14 postcoitum through week 6 postnatum was divided into the beginning of osteogenesis, woven bone, and lamellar bone stages. Versican protein was abundant in the woven bone matrix, but decreased in the lamellar bone matrix. Versican mRNA was prominent in some osteoblasts with corresponding localization of the cognate protein. The temporal and spatial mRNA expression pattern of ADAMTS1, 4, and 5 was comparable to that of versican. These results suggest that woven bone rich in versican alters into lamellar bone containing little versican during bone development in both mandibles and hind limbs, where some osteoblasts may be involved in production as well as degradation of versican by secreting ADAMTS1, 4, and 5.