Stable transformation of eggplant (Solanum melongena L.) by cocultivation of tissues withAgrobacterium tumefaciens carrying a binary plasmid vector
Stable transformation of eggplant (Solanum melongena L.) by cocultivation of tissues withAgrobacterium tumefaciens carrying a binary plasmid vector
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DOI：
10.1007/bf00716677
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发表时间：
1989-06
影响因子：
6.2
通讯作者：
Edgardo Filippone;P. Lurquin
中科院分区：
文献类型：
--
作者：
Edgardo Filippone;P. Lurquin
通过将子叶和幼叶与携带质粒pGA472的高毒力、完全致癌的根癌农杆菌菌株A281共培养，获得了茄子对卡那霉素抗性的稳定转化。使用 disarmedA 时未观察到转化。携带 pGA472 的 tumefaciensLBA4404 菌株或使用任一菌株与茄子悬浮细胞共培养时。在卡那霉素存在下生长的愈伤组织细胞上进行的 NPTII 酶和 DNA 斑点印迹分析表明外源基因的存在和表达。从完整的子叶叶片中获得最高比例的转化外植体，而在源自表面受伤的子叶叶片的愈伤组织细胞中检测到最高的NPTII酶比活性。培养六个月后，卡那霉素抗性植株再生。
Stable transformation of eggplant to kanamycin resistance was obtained by cocultivation of cotyledonary and young leaves with the hypervirulent, fully oncogenicAgrobacterium tumefaciensstrain A281 carrying plasmid pGA472. No transformation was observed when using the disarmedA. tumefaciensLBA4404 strain carrying pGA472 or when using either strain for cocultivation with eggplant suspension cells.The NPTII enzyme and DNA dot blot assays performed on callus cells growing in the presence of kanamycin indicated both the presence and expression of the foreign gene. The highest proportion of transformed explants was obtained from intact cotyledonary leaf pieces while the highest NPTII enzyme specific activity was detected in callus cells originating from superficially wounded cotyledonary leaf pieces. Kanamycin-resistant plantlets were regenerated after six months in culture.