Chemiluminescence of carbon dots induced by diperiodato-nicklate (IV) in alkaline solution and its application to a quenchometric flow-injection assays of paracetamol, L-cysteine and glutathione
Chemiluminescence of carbon dots induced by diperiodato-nicklate (IV) in alkaline solution and its application to a quenchometric flow-injection assays of paracetamol, L-cysteine and glutathione
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DOI：
10.1007/s00604-014-1427-y
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发表时间：
2015-04-01
影响因子：
5.7
通讯作者：
Sun, Hanwen
中科院分区：
文献类型：
--
作者：
Dong, Yajuan;Su, Ming;Sun, Hanwen
采用微波辅助热碳化法制备了碳量子点水溶液。用透射电镜、吸收光谱和荧光光谱对它们进行了研究。研究了强氧化剂二过碘酸镍（IV）在强碱性溶液中诱导C点化学发光的机理。提出了该反应的机理。对乙酰氨基酚、L-半胱氨酸和谷胱甘肽对体系的化学发光有猝灭作用。在优化条件下，校准曲线呈线性，相关系数（r）> 0.995。对乙酰氨基酚、L-半胱氨酸和谷胱甘肽的检测限分别为90、8和60 A μ g L-1。分析加标尿液和血清样品，回收率在84.38%-116.0%范围内，RSD为1.2%-2.7%。
Aqueous solutions of carbon dots (C-dots) were prepared by microwave-assisted thermal carbonization of poly(ethylene glycol). They were investigated by transmission electron microscopy, absorption and fluorescence spectra. It is shown that diperiodato-nicklate(IV), a strong oxidant, induces the chemiluminescence (CL) of C-dots in strongly alkaline solution without use of an additional reagent. A mechanism for this reaction is suggested. It is also found that the CL of the system is quenched by paracetamol, L-cysteine and glutathione. Under the optimized conditions, the calibration plot is linear with a correlation coefficient (r) of > 0.995. The limits of detection are 90, 8, and 60 A mu g L-(1) for paracetamol, L-cysteine, and glutathione, respectively. Spiked urine and serum samples were analyzed and gave recoveries in the range from 84.38 to 116.0 %, with an RSD of 1.2-2.7 %.