Corticotropin-releasing hormone-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP) are expressed in the gills and skin of common carp Cyprinus carpio L. and respond to acute stress and infection
Corticotropin-releasing hormone-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP) are expressed in the gills and skin of common carp Cyprinus carpio L. and respond to acute stress and infection
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DOI：
10.1242/jeb.01973
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发表时间：
2006-02-01
影响因子：
2.8
通讯作者：
Huising, MO
中科院分区：
文献类型：
--
作者：
Mazon, AF;Kemenade, BMLV;Huising, MO
我们证实了促肾上腺皮质激素释放激素（CRH）、CRH结合蛋白（CRH- bp）和CRH受体1 （CRH- r1）在早期脊椎动物鲤鲤（Cyprinus carpio）的鳃和皮肤中表达。在鳃和皮肤的巨噬细胞样细胞、皮肤的成纤维细胞和鳃的内皮细胞中检测到免疫反应性CRH。CRH系统在鳃和皮肤的参与研究了对感染的反应和急性约束应激范式。用水蛭传播的血鞭毛虫伯氏锥虫感染鲫鱼，用网捕法进行急性抑制应激24h。感染和抑制后，鳃和皮肤中CRH-BP和CRH-R1基因的表达均下调。因此外周CRH系统对感染和压力作出反应。鳃和皮肤将内部环境与外部环境分开，并永久暴露在压力和病原体中。由于这些器官在维持体内平衡中起着关键作用，它们必须局部行动以应对不同的压力。显然，CRH系统参与了内部和外部环境的脆弱界面对不同应力的响应。
We established that corticotropin-releasing hormone (CRH), CRH-binding protein (CRH-BP) and CRH-receptor 1 (CRH-R1) are expressed in the gills and skin of common carp Cyprinus carpio, an early vertebrate. Immunoreactive CRH was detected in macrophage-like cells in gills and skin, in fibroblasts in the skin and in endothelial cells in the gills. The involvement of the CRH system in gills and skin was investigated in response to infection and in an acute restraint stress paradigm. Carp were infected with the protozoan leech-transmitted blood flagellate Trypanoplasma borreli and subjected to acute restraint stress by netting for 24h. The expression of CRH-BP and CRH-R1 genes in the gills and in the skin is downregulated after both infection and restraint. Thus the peripheral CRH system reacts to infection and stress. The gills and skin separate the internal from the external environment and are permanently exposed to stress and pathogens. Because of their pivotal role in maintaining the homeostatic equilibrium, these organs must act locally to respond to diverse stresses. Clearly, the CRH system is involved in the response of the integument to diverse stresses at the vulnerable interface of the internal and external milieu.