An Arabidopsis DISEASE RELATED NONSPECIFIC LIPID TRANSFER PROTEIN 1 is required for resistance against various phytopathogens and tolerance to salt stress
An Arabidopsis DISEASE RELATED NONSPECIFIC LIPID TRANSFER PROTEIN 1 is required for resistance against various phytopathogens and tolerance to salt stress
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DOI：
10.1016/j.gene.2020.144802
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发表时间：
2020-08-30
期刊：
影响因子：
3.5
通讯作者：
Raina, Ramesh
中科院分区：
文献类型：
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作者：
Dhar, Nikhilesh;Caruana, Julie;Raina, Ramesh
多个基因的同步和及时调控导致有效的防御反应，当受到病原体或环境条件意外变化的挑战时，决定宿主的命运。一个这样的基因，这是下调多种细菌病原体，是一个假定的非特异性脂质转移蛋白（nsLTP）的未知功能，我们命名为疾病相关的非特异性脂质转移蛋白1（DRN 1）。我们发现，在病原体的挑战，DRN 1是强烈下调，而一个假定的DRN 1靶向新的microRNA（miRNA）命名为DRN 1调节miRNA（DmiR）是上调。此外，我们提供的证据表明，DRN 1是防御细菌和真菌病原体以及盐胁迫下的正常幼苗生长所需的。虽然已知来自不同植物物种的nsLTP家族成员是食物过敏原的重要来源，并且通常与抗菌特性相关，但我们对该基因家族的生物学功能和调控的了解有限。我们目前的工作不仅揭示了调控机制，而且有助于DRN 1的功能表征，DRN 1是迄今未知功能的推定nsLTP家族成员。
Synchronous and timely regulation of multiple genes results in an effective defense response that decides the fate of the host when challenged with pathogens or unexpected changes in environmental conditions. One such gene, which is downregulated in response to multiple bacterial pathogens, is a putative nonspecific lipid transfer protein (nsLTP) of unknown function that we have named DISEASE RELATED NONSPECIFIC LIPID TRANSFER PROTEIN 1 (DRN1). We show that upon pathogen challenge, DRN1 is strongly downregulated, while a putative DRN1-targeting novel microRNA (miRNA) named DRN1 Regulating miRNA (DmiR) is reciprocally upregulated. Furthermore, we provide evidence that DRN1 is required for defense against bacterial and fungal pathogens as well as for normal seedling growth under salinity stress. Although nsLTP family members from different plant species are known to be a significant source of food allergens and are often associated with antimicrobial properties, our knowledge on the biological functions and regulation of this gene family is limited. Our current work not only sheds light on the mechanism of regulation but also helps in the functional characterization of DRN1, a putative nsLTP family member of hitherto unknown function.