Self-assembled nanoparticle probes for recognition and detection of biomolecules
Self-assembled nanoparticle probes for recognition and detection of biomolecules
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DOI：
10.1021/ja025814p
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发表时间：
2002-08-14
影响因子：
15
通讯作者：
Nie, SM
中科院分区：
文献类型：
--
作者：
Maxwell, DJ;Taylor, JR;Nie, SM
胶体金纳米晶体已被用于开发一种新的纳米传感器，该纳米传感器能够以均匀格式识别和检测特定的DNA序列和单基碱基突变。该生物传感器的核心是2.5 nm的金纳米颗粒，既可以用作纳米折叠剂和纳米淬火器（有效的能量受体）。附着在该核心上的是寡核苷酸分子在一端用硫醇组标记，另一端标记为荧光团。发现这种杂交生物/无机构建体可自发地组装成颗粒表面上受约束的拱形构象。靶分子的结合会导致构象变化，从而恢复淬灭荧光团的荧光。与具有茎和环结构的常规分子信标不同，纳米颗粒探针不需要茎，并且其背景荧光随温度的增加很小。与有机淬灭剂（4,4'-二甲基氨基苯基苯甲酸苯甲酸）相比，金属纳米颗粒具有独特的结构和光学特性，用于新应用于生物透射和分子工程。
Colloidal gold nanocrystals have been used to develop a new class of nanobiosensors that is able to recognize and detect specific DNA sequences and single-base mutations in a homogeneous format. At the core of this biosensor is a 2.5-nm gold nanoparticle that functions as both a nano-scaffold and a nano-quencher (efficient energy acceptor). Attached to this core are oligonucleotide molecules labeled with a thiol group at one end and a fluorophore at the other. This hybrid bio/inorganic construct is found to spontaneously assemble into a constrained arch-like conformation on the particle surface. Binding of target molecules results in a conformational change, which restores the fluorescence of the quenched fluorophore. Unlike conventional molecular beacons with a stem-and-loop structure, the nanoparticle probes do not require a stem, and their background fluorescence increases little with temperature. In comparison with the organic quencher Dabcyl (4,4'-dimethylaminophenyl azo benzoic acid), metal nanoparticles have unique structural and optical properties for new applications in biosensing and molecular engineering.