Effects of indole-3-carbinol and phenethyl isothiocyanate on bile and pancreatic juice excretion in rats.
Effects of indole-3-carbinol and phenethyl isothiocyanate on bile and pancreatic juice excretion in rats.
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DOI：
10.2152/jmi.59.246
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
H. Ishibashi;T. Kuwahara;H. Nakayama-Imaohji;Y. Ohnishi;H. Mori;M. Shimada
中科院分区：
文献类型：
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作者：
H. Ishibashi;T. Kuwahara;H. Nakayama-Imaohji;Y. Ohnishi;H. Mori;M. Shimada
胆汁和胰液含有许多癌症化学预防的参数。吲哚-3-甲醇（I3 C）和异硫氰酸苯乙酯（PEITC）是芸苔属植物的水解产物，已被确定为抗癌剂。本研究建立了一种连续、选择性胆汁和胰液取样方法，并研究了I3 C和PEITC对胆汁和胰液排泄及样品中γ-谷氨酰转肽酶（γ-GTP）活性的影响。雄性Fisher 344大鼠（8周龄）用I3 C（150 mg/kg）或PEITC（160 mg/kg）灌胃激发5天。最后一次给药后24 h，在大鼠胆管和胰管内插管，分别收集胆汁和胰液48 h。在该大鼠模型中，胆汁排泄稳定，对照组大鼠的胆汁和胰腺排泄量分别为21.9 ± 1.4 ml/48 h和12.8 ± 1.7 ml/48 h。与对照组大鼠相比，I3 C或PEITC治疗组大鼠前24小时的胆汁排泄量显著增加。在胰液的情况下，PEITC处理的大鼠在前24小时内的排泄量显著增加。在胆汁中，γ-GTP活性在I3 C和PEITC处理的大鼠中在前24 h显著增加，但在胰液中没有观察到差异。I3 C和PEITC的抗肿瘤作用可能与胆汁中γ-GTP活性和胆汁排泄增加有关。本文所述的大鼠模型是研究癌症化学预防的有用工具。
Bile and pancreatic juice contain a number of parameters for cancer chemoprevention. Indole-3-carbinol (I3C) and phenethyl isothiocyanate (PEITC), which are hydrolytic products of brassica plants, have been established to be anti-cancer agents. Here, we developed a method for the continuous and selective sampling of bile and pancreatic juice, and the effects of I3C and PEITC on bile and pancreatic excretion and γ-glutamyl transpeptidase (γ-GTP) activity in the samples were investigated. Male Fisher 344 rats (eight weeks of age) were challenged intragastrically with I3C (150 mg/kg) or PEITC (160 mg/kg) for five days. Twenty-four hours after the final administration, cannulation was undertaken into the rats' bile and pancreatic ducts, and the bile and pancreatic juice were separately collected for 48 h. In this rat model, bile was stably excreted, and the bile and pancreatic excretion of the control rats was 21.9 ± 1.4 ml/48 h and 12.8 ± 1.7 ml/48 h, respectively. Bile excretion for the first 24 h significantly increased in the I3C- or PEITC-treated rats compared with the control rats. In the case of pancreatic juice, excretion during the first 24 h significantly increased in the PEITC-treated rats. In bile, γ-GTP activity was significantly increased for the first 24 h in the I3C- and PEITC-treated rats, but no difference was observed in the pancreatic juice. Increases of bile excretion and γ-GTP activity in bile might be a factor involved in the anti-cancer effect of I3C and PEITC. Our rat model described here is a useful tool for the study of cancer chemoprevention.