Association of Oral Microbiome With Risk for Incident Head and Neck Squamous Cell Cancer
Association of Oral Microbiome With Risk for Incident Head and Neck Squamous Cell Cancer
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DOI：
10.1001/jamaoncol.2017.4777
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发表时间：
2018-03-01
期刊：
影响因子：
28.4
通讯作者：
Pei, Zhiheng
中科院分区：
文献类型：
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作者：
Hayes, Richard B.;Ahn, Jiyoung;Pei, Zhiheng
病例对照研究表明口腔细菌与头颈部鳞状细胞癌（HNSCC）之间可能存在关系。前瞻性研究需要检查口腔微生物组和随后的HNSCC.Objective风险之间的时间关系，以前瞻性地检查口腔微生物组和事件HNSCC.DESIGN之间的关联，设置，和参与者这项巢式病例对照研究在2个前瞻性队列研究中进行：美国癌症协会癌症预防研究II营养队列（CPS-II）和前列腺癌、肺癌、结直肠癌和卵巢癌筛查试验（PLCO）。在122004名参与者中，在平均3.9年的随访期间确定了129例HNSCC事件患者病例。通过发病率密度采样，在年龄、性别、人种/种族和漱口水采集后的时间上进行匹配，每例患者病例（n = 254）选择两个对照组。所有参与者都提供了漱口水样本，并且在基线时没有癌症。分别使用PERMANOVA和负二项广义线性模型比较病例组和对照组的总体口腔微生物组组成和特定分类群丰度，控制年龄，性别，种族，队列，吸烟，酒精和口腔人乳头瘤病毒-16状态。具有双侧错误发现率（FDR）校正P值（q值）的分类群
IMPORTANCE Case-control studies show a possible relationship between oral bacteria and head and neck squamous cell cancer (HNSCC). Prospective studies are needed to examine the temporal relationship between oral microbiome and subsequent risk of HNSCC.OBJECTIVE To prospectively examine associations between the oral microbiome and incident HNSCC.DESIGN, SETTING, AND PARTICIPANTS This nested case-control study was carried out in 2 prospective cohort studies: the American Cancer Society Cancer Prevention Study II Nutrition Cohort (CPS-II) and the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO). Among 122 004 participants, 129 incident patient cases of HNSCC were identified during an average 3.9 years of follow-up. Two controls per patient case (n = 254) were selected through incidence density sampling, matched on age, sex, race/ethnicity, and time since mouthwash collection. All participants provided mouthwash samples and were cancer-free at baseline.EXPOSURES Oral microbiome composition and specific bacterial abundances were determined through bacterial 16S rRNA gene sequencing. Overall oral microbiome composition and specific taxa abundances were compared for the case group and the control group, using PERMANOVA and negative binomial generalized linear models, respectively, controlling for age, sex, race, cohort, smoking, alcohol, and oral human papillomavirus-16 status. Taxa with a 2-sided false discovery rate (FDR)-adjusted P value (q-value)