Measuring Tumor Glycolytic Flux in Vivo by Using Fast Deuterium MRI
Measuring Tumor Glycolytic Flux in Vivo by Using Fast Deuterium MRI
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DOI：
10.1148/radiol.2019191242
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发表时间：
2020-02-01
期刊：
影响因子：
19.7
通讯作者：
Brindle, Kevin M.
中科院分区：
文献类型：
--
作者：
Kreis, Felix;Wright, Alan J.;Brindle, Kevin M.
背景资料：肿瘤细胞经常表现出高速率的有氧糖酵解，这提供了细胞快速生长和增殖的增加的生物合成需求所需的糖酵解中间体。现有临床方法（氟脱氧葡萄糖PET和碳13 MRI和光谱学）不允许糖酵解通量的定量图像。（氢2 [H-2]）MR光谱成像用于肿瘤糖酵解通量的定量映射和评估对化疗的反应。快速三维H-2 MR光谱成像脉冲序列，时间分辨率为10分钟，用于在团注D-[6，6 ′-H-2（2）]葡萄糖。肿瘤乳酸标记，表示为乳酸-水和乳酸-葡萄糖信号比，也进行了评估，在本地化H-2 MR光谱。统计学显著性进行了测试与单侧配对t test.Results：H-2磁共振波谱成像显示异质性糖酵解通量在整个肿瘤和早期减少通量与化疗药物治疗后。对5只动物的光谱测量显示乳酸盐与水的信号比从0.33 +/- 0.10降低到0.089 +/- 0.039（P = 0.005），乳酸/葡萄糖比值从0.27 +/- 0.12至0.12 +/- 0.06结论：快速获取的氘（氢2）MR光谱图像可以提供定量和空间分辨测量的糖酵解通量的肿瘤，可用于评估治疗反应。在CC BY 4.0许可证下发布。
Background: Tumor cells frequently show high rates of aerobic glycolysis, which provides the glycolytic intermediates needed for the increased biosynthetic demands of rapid cell growth and proliferation. Existing clinical methods (fluorodeoxyglucose PET and carbon 13 MRI and spectroscopy) do not allow quantitative images of glycolytic flux.Purpose: To evaluate the use of deuterium (hydrogen 2 [H-2]) MR spectroscopic imaging for quantitative mapping of tumor glycolytic flux and to assess response to chemotherapy.Materials and Methods: A fast three-dimensional H-2 MR spectroscopic imaging pulse sequence, with a time resolution of 10 minutes, was used to image glycolytic flux in a murine tumor model after bolus injection of D-[6,6'-H-2(2)]glucose before and 48 hours after treatment with a chemotherapeutic agent. Tumor lactate labeling, expressed as the lactate-to-water and lactate-to-glucose signal ratios, was also assessed in localized H-2 MR spectra. Statistical significance was tested with a one-sided paired t test.Results: H-2 MR spectroscopic imaging showed heterogeneity in glycolytic flux across the tumor and an early decrease in flux following treatment with a chemotherapeutic drug. Spectroscopy measurements on five animals showed a decrease in the lactate-to-water signal ratio, from 0.33 +/- 0.10 to 0.089 +/- 0.039 (P =.005), and in the lactate-to-glucose ratio, from 0.27 +/- 0.12 to 0.12 +/- 0.06(P = .04), following drug treatment.Conclusion: Rapidly acquired deuterium (hydrogen 2) MR spectroscopic images can provide quantitative and spatially resolved measurements of glycolytic flux in tumors that can be used to assess treatment response. Published under a CC BY 4.0 license.