A polymer scaffold for protein oligomerization
A polymer scaffold for protein oligomerization
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DOI：
10.1021/ja037646m
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发表时间：
2004-02-18
影响因子：
15
通讯作者：
Kiessling, LL
中科院分区：
文献类型：
--
作者：
Griffith, BR;Allen, BL;Kiessling, LL
我们报道了用于蛋白质非共价齐聚的明确定义的聚合物的设计和合成。已报道的支架是通过原子转移自由基聚合(ATRP)产生的，利用了Ni2+络合物和寡组氨酸序列(His Tag)之间的良好相互作用。因此，我们的聚合物旨在促进任何具有组氨酸标签的蛋白质的齐聚。我们证明，在无肝素的情况下，它们可以寡聚成纤维细胞生长因子-8b(FGF-8b)并促进成纤维细胞生长因子-8b介导的细胞增殖。
We report the design and synthesis of well-defined polymers for the noncovalent oligomerization of proteins. The reported scaffolds, which were generated by atom-transfer radical polymerization (ATRP), take advantage of the well-characterized interaction between a Ni2+complex and an oligohistidine sequence (His tag). Thus, our polymers are designed to facilitate the oligomerization of any protein possessing a His tag. We demonstrate that they can oligomerize fibroblast growth factor-8b (FGF-8b) and promote FGF-8b-mediated cell proliferation in the absence of heparin.