Season's Variation Impact on Citrus aurantium Leaves Essential Oil: Chemical Composition and Biological Activities
Season's Variation Impact on Citrus aurantium Leaves Essential Oil: Chemical Composition and Biological Activities
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DOI：
10.1111/j.1750-3841.2012.02846.x
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发表时间：
2012-09-01
影响因子：
3.9
通讯作者：
Bouajila, Jalloul
中科院分区：
文献类型：
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作者：
Ellouze, Ines;Abderrabba, Manef;Bouajila, Jalloul
对柑橘叶精油(Eos)的化学成分及抗氧化、抑菌活性进行了研究。植物原料在一年中采集了5次，经过水蒸气蒸馏制备环氧乙烷。通过气相色谱/质谱联用和GC/火焰离子化检测进行化学表征，可以鉴定出46种化合物，不同收获期的不同小粒样品之间存在显著的定量和定性差异。主要成分为芳樟醇(43.2%~65.97%)、乙酸芳樟酯(0.77%~24.77%)和α-松油醇(9.29%~12.12%)。最重要的组成部分数量登记为夏季状态观测系统(7月和9月)。这5个EOS提交了生物活性筛选，即抗氧化和抗菌活性。1，1-二苯基-2-苦基肼和2，2‘-azinobis-3-ethylbenzothiazoline-6-sulphonate法均检测到较弱的抗氧化活性(IC_(50)值>；10000 mg/L)，这主要是由于EOS中酚的含量较低所致。对革兰氏阳性菌[枯草芽孢杆菌(MIC)2.7 mg/m L，金黄色葡萄球菌(4.8 m g/m L)]，对酵母菌[酿酒酵母(9.2 m g/m l)]和真菌[拉曼毛霉(M Ucor Ramannianus)(5 m g/m L)]的抑菌活性较弱。已鉴定的化合物与抗菌活性之间存在正相关关系。许多化合物都与抑菌活性有关，主要是石竹烯氧化物对大肠杆菌(R2=0.99)、酿酒葡萄球菌(R2=0.99)和镰刀菌(R2=0.99)的抑制作用。
Citrus aurantium leaves' essential oils (EOs) were evaluated for chemical composition and antioxidant and antibacterial activities. The vegetable material, taken 5 times during the year, has undergone the hydrodistillation to prepare EO. Chemical characterization by gas chromatography/mass spectrometry and GC/flame ionization detection allowed the identification of 46 compounds, and a notable quantitative and qualitative differences between the different Petitgrain samples according to the harvest time. Linalool (43.2% to 65.97%), linalyl acetate (0.77% to 24.77%), and a-terpineol (9.29% to 12.12%) were the main components. The most important number of components was registered for summer EOs (July and September). The 5 EOs submitted biological activities screening, namely, antioxidant and antimicrobial activities. Weak antioxidant activities (IC50 values >10000 mg/L) were registered by both 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate assays, mostly because the weak amount of phenols in EOs. Antibacterial activities (12 microorganisms) were registered against Gram-positive bacteria [Bacillus subtilis (MIC = 2.7 mg/mL), Staphylococcus aureus (4.8 mg/mL)], and moderated ones against yeasts [Saccharomyces cerevisiae (9.2 mg/mL)] and fungi [Mucor ramannianus (5 mg/mL)]. Positive correlations between the identified compounds and the antimicrobial activities were noted. Many compounds were correlated to antimicrobial activity mainly caryophyllene oxide against Escherichia coli (R2= 0.99), S. cerevisiae (R2= 0.99), and Fusarium culmorum (R2= 0.99).