Ontogeny of mesenchymal androgen receptors in the embryonic mouse mammary gland.
Ontogeny of mesenchymal androgen receptors in the embryonic mouse mammary gland.
复制标题
胚胎小鼠乳腺间充质雄激素受体的个体发育。
DOI：
10.1210/endo-113-5-1771
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
Klaus Kratochwil
中科院分区：
文献类型：
--
作者：
G. Wasner;Ilse Hennermann;Klaus Kratochwil
我们证明了胚胎小鼠乳腺中存在一个高亲和力的雄激素结合部位，并描述了它的出现和发育，从最初的腺芽形成(第12天)到雄激素反应阶段(第14天)到足月(第19天)。在体外对暴露于配体[(~3H]睾酮、[~H]5-α-二氢睾酮或[~3H]甲基三烯醇酮)的整个器官雏形进行结合分析，并表达每个腺体的受体水平。在竞争实验中，结合部位显示出典型的啮齿动物雄激素受体的配基特异性。Scatchard分析得出一个14天的腺体有9000-1亿个结合位点，或者说每个靶细胞大约有30,000个结合位点。与完整组织结合的配体的表观解离常数Kd在0.55-0.75×10(-9)M之间。第一个结合部位在乳芽形成时(第12天)变得可检测到；它们的数量在反应阶段(第14天)增加了约20倍，并至少在出生前保持在高水平。第15天雄激素反应性的丧失既不伴随受体的丧失，也不伴随其结合亲和力的改变。
We demonstrate the presence of a high affinity androgen-binding site in the embryonic mouse mammary gland, and describe its appearance and development from the initial formation of the gland bud (day 12) through the androgen-responsive stage (day 14) until term (day 19). Binding assays were done on entire organ rudiments exposed to the ligand [( 3H] testosterone, [3H]5 alpha-dihydrotestosterone, or [3H]methyltrienolone) in vitro, and receptor levels are expressed per gland. In competition experiments, the binding site shows a ligand specificity of a typical rodent androgen receptor. Scatchard analysis yielded a figure of 90-100 million binding sites for one 14-day gland, or approximately 30,000 per target cell. The apparent dissociation constant Kd for ligand binding by the intact tissue was between 0.55 and 0.75 X 10(-9) M. The first binding sites become detectable at the time of mammary bud formation (day 12); their number increases about 20-fold towards the responsive stage (day 14), and they persist at high levels at least until birth. The loss of androgen responsiveness on day 15 is neither accompanied by a loss of receptors nor by a change of their binding affinity.
钼酸钠对小鼠肾脏中雄激素受体的稳定作用的表征。
影响因子：
4.8
作者：
Wright,WW;Chan,KC;Bardin,CW
通讯作者：
Bardin,CW