Excitotoxicity Plays a Role in the Death of Tyrosine Hydroxylase- Immunopositive Nigral Neurons Cultured in Serum-Free Medium
Excitotoxicity Plays a Role in the Death of Tyrosine Hydroxylase- Immunopositive Nigral Neurons Cultured in Serum-Free Medium
复制标题
兴奋性毒性在无血清培养基中培养的酪氨酸羟化酶免疫阳性黑质神经元死亡中发挥作用
作者：
G. Schierle;P. Brundin
我们研究了不同氨基酸受体拮抗剂和钙（Ca2+）通道阻滞剂对无血清培养条件下生长的胚胎酪氨酸羟化酶（TH）免疫阳性黑质神经元存活的影响。腹侧中脑神经元培养2、7天。在第2天停用血清后，用不同浓度的非竞争性n -甲基-d -天冬氨酸（NMDA）受体拮抗剂二唑西平氢马来酸（MK-801）、竞争性NMDA受体拮抗剂2-氨基-5-磷酸戊酸、竞争性海碱盐/准酸盐受体拮抗剂6,7-二硝基喹啉- 2,3 -二酮和Ca2+通道阻滞剂氟桂利嗪处理一些培养物。用MK-801或氟桂利嗪治疗可提高血清剥夺后th阳性神经元的存活率。这些发现表明兴奋毒性在多巴胺能细胞死亡中的可能作用，可以通过阻断NMDA受体或抑制Ca2+通过电压门控通道进入来预防。
We studied the effects of different amino acid receptor antagonists and a calcium (Ca2+) channel blocker on the survival of embryonic tyrosine hydroxylase (TH)-immunopositive nigral neurons grown under serum-free culture conditions. Ventral mesencephalic neurons were cultivated for 2 or 7 days. Following serum withdrawal on day 2, some cultures were treated with different concentrations of the noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine hydrogen maleate (MK-801), the competitive NMDA receptor antagonist 2-amino-5-phosphonopentanoic acid, the competitive kainate/quisqualate receptor antagonist 6,7-dinitroquinoxaline-2, 3-dione, and the Ca2+ channel blocker flunarizine. Treatment with MK-801 or flunarizine increased the survival of TH-positive neurons after serum deprivation. These findings suggest a possible role for excitotoxicity in dopaminergic cell death which can be prevented by blocking the NMDA receptor or by inhibiting Ca2+ entry through voltage-gated channels.
神经元坏死和细胞凋亡：暴露于谷氨酸或氧化应激诱导的两种不同事件。
DOI：
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发表时间：
1997
期刊：
Advances in neurology.
影响因子：
--
作者：
Nicotera,P;Ankarcrona,M;Bonfoco,E;Orrenius,S;Lipton,SA
通讯作者：
Lipton,SA
红藻氨酸调节 Ca 流入纹状体神经元。
DOI：
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发表时间：
1989
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Murphy,SN;Miller,RJ
通讯作者：
Miller,RJ