Bifidobacteria can protect from enteropathogenic infection through production of acetate
Bifidobacteria can protect from enteropathogenic infection through production of acetate
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DOI：
10.1038/nature09646
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发表时间：
2011-01-27
期刊：
影响因子：
64.8
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
人类肠道中寄生着各种各样的微生物，包括对人体生理和病理有有益影响的双歧杆菌属细菌。双歧杆菌最显著的好处是调节宿主防御反应和预防传染病(4-6)。然而，这些效应背后的分子机制几乎没有被阐明。为了研究这些机制，我们使用了与某些双歧杆菌菌株相关的小鼠和一个简化的致死性肠出血性大肠杆菌O157：H7感染模型，并结合了一种集成的“组学”方法。在这里，我们证明了编码存在于某些双歧杆菌中的ATP结合盒类型碳水化合物转运体的基因有助于保护小鼠免受大肠杆菌O157：H7诱导的死亡。我们发现，这种效果可以归因于，至少在一定程度上，增加了醋酸盐的产生，并抑制了大肠杆菌O157：H7志贺毒素从肠腔到血液的转位。我们认为，保护性双歧杆菌产生的醋酸盐可以改善上皮细胞介导的肠道防御，从而保护宿主免受致命感染。
The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology(1-3). Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases(4-6). Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated 'omics' approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.