The MYB Transcription Factor Family Genes in Sugarcane (Saccharum sp.)
The MYB Transcription Factor Family Genes in Sugarcane (Saccharum sp.)
复制标题
DOI：
10.1007/s11105-014-0768-3
复制
发表时间：
2015-06-01
影响因子：
2.1
通讯作者：
Prabu, Gajjeraman
中科院分区：
文献类型：
--
作者：
Geethalakshmi, Sundararaman;Barathkumar, Sadasivam;Prabu, Gajjeraman
转录因子是控制植物生理生化过程的一类蛋白质。MYB蛋白家族是最大的转录因子（TF）家族，在控制生长发育和应激反应中起重要作用。这些基因在许多双子叶和单子叶植物中得到了广泛的研究。在禾本科植物中，甘蔗具有复杂的多倍体基因组，关于MYB TF家族的信息很少。随着该生物燃料作物的重要性日益增加，计算预测了57个R2R3-MYB， 20个myb样基因和1 3R1-MYB基因，并通过RT-PCR分离了51个scmyb基因。MYB结构域外的亚群特异性保守基序在甘蔗中显示了它们的系统发育和功能保护。根据其生物学过程，分离的ScMYB基因在功能上可分为细胞生长和维持（39.74%）、应激反应（33.33%）、代谢（14.10%）和发育调节（12.82%）。逆转录-定量聚合酶链反应（RT-qPCR）的表达分析显示，ScMYB26/AS1 >、ScMYB54 >、ScMYB66 >、ScMYB75转录本在水分亏缺条件下有显著升高的表达趋势，而在盐胁迫条件下，ScMYB1和ScMYB58在胁迫12天（DOS）期间在甘蔗叶片中有较高的表达。此外，胁迫响应型ScMYB26/AS1在烟草中通过农业浸润介导的瞬时表达进行功能表征。半qrt - pcr结果显示，在胁迫诱导过程中，导入的ScMYB26/AS1转录物在转化烟草外植体中的表达增加。总之，我们的比较基因组学分析和从甘蔗中分离出的ScMYB基因表明，通过转基因方法改善农作物的抗逆性是可行的，未来的坚实基础。
Transcription factors are a family of proteins that control the physiological and biochemical process of a plant. The MYB family of proteins is the largest family of transcription factors (TF) that plays an important role in controlling growth and development and stress responses. These genes have been extensively studied in many dicot and monocot plants. Among the grasses, sugarcane has a complex polyploidy genome, and only little information is available about the MYB TF family. With increasing importance of this biofuel crop, 57 R2R3-MYB, 20 MYB-like, and 1 3R1-MYB genes were computationally predicted and 51 ScMYBs were isolated by RT-PCR. Subgroup-specific conserved motifs outside the MYB domain demonstrated their phylogeny and functional conservation in sugarcane. Based on their biological process, the isolated ScMYB genes were functionally categorized into cell growth and maintenance (39.74 %), stress response (33.33 %), metabolism (14.10 %), and developmental regulation (12.82 %). Expression analysis by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) revealed a significantly higher expression trend of ScMYB26/AS1 > ScMYB54 > ScMYB66 > ScMYB75 transcripts in response to water deficit, whereas in salt stress, ScMYB1 and ScMYB58 showed higher expression during 12 days of stress (DOS) in sugarcane leaves. Further, the stress-responsive ScMYB26/AS1 was functionally characterized by agroinfiltration-mediated transient expression in tobacco. Semi-qRT-PCR demonstrated the increased expression of introduced ScMYB26/AS1 transcript in transformed tobacco explants during stress induction. Taken together, our comparative genomics analysis and isolation of ScMYB genes from sugarcane illustrated the viable, future solid foundation for improvement of agricultural crops to stress tolerance by a transgenic approach.