Improvement of bacterial blight resistance of 'Minghui 63', an elite restorer line of hybrid rice, by molecular marker-assisted selection
Improvement of bacterial blight resistance of 'Minghui 63', an elite restorer line of hybrid rice, by molecular marker-assisted selection
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DOI：
10.2135/cropsci2000.401239x
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发表时间：
2000-01-01
期刊：
影响因子：
2.3
通讯作者：
Zhang, QF
中科院分区：
文献类型：
--
作者：
Chen, S;Lin, XH;Zhang, QF
‘明恢63’是中国杂交水稻生产中广泛使用的恢复系。然而，该品系已变得越来越容易受到白叶枯病（BB）的影响，导致其在水稻生产中的使用迅速下降。采用分子标记辅助选择（MAS）方法，将广谱BE抗性基因Xa21导入明会63，提高其对BE的抗性。建立了基于聚合酶链反应（PCR）的MAS系统，该系统由Xa21的一部分组成，标记位于距Xa21位点0.8 cM的一侧，标记位于距基因3.0 cM的另一侧。利用均匀分布在12条染色体上的128个限制性片段长度多态性（RFLP）标记对明辉63的遗传背景进行了恢复，得到的改良版本“明辉63（Xa21）”除了在Xa21位点周围有一个长度小于3.8 cM的片段外，与原始版本完全相同。明会63（Xa21）及其与真山97A杂交的“汕优63（Xa21）”均表现出与供体亲本相同的BE抗性谱。田间农艺性状鉴定表明，在无病害胁迫条件下，明恢63（Xa21）和汕优63（Xa21）与明恢63和汕优63具有相同的性状。在重病条件下，明恢63（Xa21）的粒重和穗肥力显著高于明恢63，汕优63（Xa21）的每穗粒数、粒重和产量显著高于汕优63。
'Minghui 63' is a restorer line widely used in hybrid rice production in China. However, this line has become increasingly susceptible to bacterial blight (BB), resulting in a rapid decline of its use in rice production. The objective of this study was to improve the BE resistance of Minghui 63 by introgressing Xa21, a broad-spectrum BE resistance gene, into Minghui 63 by molecular marker-assisted selection (MAS). A polymerase chain reaction (PCR)-based MAS system was established consisting of a marker that is a part of Xa21, a marker located at 0.8 centimorgans (cM) from the Xa21 locus on one side, and a marker at 3.0 cM from the gene on the other side. A total of 128 restriction fragment length polymorphism (RFLP) markers, evenly distributed on the 12 chromosomes, were used to recover the genetic background of Minghui 63, The resulting improved version of Minghui 63, or 'Minghui 63(Xa21)', was exactly the same as the original except for a fragment of less than 3.8 cM in length surrounding the Xa21 locus. Both Minghui 63(Xa21) and its hybrid with 'Zhenshan 97A' referred to as 'Shanyou 63(Xa21)' showed the same spectrum of BE resistance as the donor parent. Field examination of a number of agronomic traits showed that Minghui 63(Xa21) and Shanyou 63(Xa21) were identical to Minghui 63 and Shanyou 63, when there was no disease stress. Under heavily diseased conditions, Minghui 63(Xa21) showed significantly higher grain weight and spikelet fertility than Minghui 63, and Shanyou 63(Xa21) was significantly higher than Shanyou 63 in grains per panicle, grain weight, and yield.