Acinetobacter brisouii sp nov., Isolated from a Wetland in Korea
Acinetobacter brisouii sp nov., Isolated from a Wetland in Korea
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DOI：
10.1007/s12275-009-0132-8
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发表时间：
2010-02-01
影响因子：
3
通讯作者：
Kwon, Soon-Wo
中科院分区：
文献类型：
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作者：
Anandham, Rangasamy;Weon, Hang-Yeon;Kwon, Soon-Wo
从韩国永内县泥炭层中分离到一株细菌5YN5-8(T)。菌株5YN5-8(T)的细胞是严格需氧的，革兰氏阴性，球状杆菌，无芽胞形成，不能移动。该菌株最适生长温度为28℃，最适pH为7.0，最适盐度为0-1%。16S rRNA基因序列分析表明，该菌株与醋酸钙不动杆菌有较近的亲缘关系(与菌株DSM 30006(T)的相似性为97.8%)。与已发表的不动杆菌16S rRNA基因序列同源性为94.4-97.8%。5YN5-8(T)菌株与不动杆菌属其他成员的DNA-DNA杂交值为16%~28%。主要的细胞脂肪酸是C-18：1 omega 9c，包含C-15：0 iso2-OH和/或C-16：1 omega 7c的特征4和C-16：0。DNA G+C含量为43.9mol%。本研究积累的系统发育、表型和化学分类数据表明，该分离株可归入不动杆菌属的一个新种。命名为布氏不动杆菌。11月5YN5-8(T)(=KAcc 11602(T)=Dsm 18516(T))为模式菌株。
A bacterial strain 5YN5-8(T) was isolated from peat layer on Yongneup in Korea. Cells of strain 5YN5-8(T) were strictly aerobic, Gram-negative, coccobacilli, non-spore forming, and non-motile. The isolate exhibited optimal growth at 28 degrees C, pH 7.0, and 0-1% NaCl. Results of 16S rRNA gene sequence analyses indicated a close relationship of this isolate to Acinetobacter calcoaceticus (97.8% similarity for strain DSM 30006(T)). It also exhibited 94.4-97.8% 16S rRNA gene sequence similarities to the validly published Acinetobacter species. The value for DNA-DNA hybridization between strain 5YN5-8(T) and other members of the genus Acinetobacter ranged from 16 to 28%. Predominant cellular fatty acids were C-18:1 omega 9c, summed feature 4 containing C-15:0 iso 2-OH and/or C-16:1 omega 7c, and C-16:0. The DNA G+C content was 43.9 mol%. Phylogenetic, phenotypic, and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Acinetobacter. The name Acinetobacter brisouii sp. nov. is proposed for the novel species, with 5YN5-8(T) (=KACC 11602(T) = DSM 18516(T)) as the type strain.