Influence of maternal stress on the effects of prenatal exposure to methylmercury and arsenic on postnatal development and behavior in mice: A preliminary evaluation
Influence of maternal stress on the effects of prenatal exposure to methylmercury and arsenic on postnatal development and behavior in mice: A preliminary evaluation
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DOI：
10.1016/s0031-9384(96)00462-3
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发表时间：
1997-03-01
影响因子：
2.9
通讯作者：
Corbella, J
中科院分区：
文献类型：
--
作者：
Colomina, MT;Albina, ML;Corbella, J
本研究将母体束缚应激与暴露于两种环境有毒元素（汞和砷）相结合，在束缚期（妊娠第15 - 18天的1000 - 1200小时）同时给予小鼠。两组动物通过灌胃给予氯化甲基汞（MMC）（2毫克/千克/天），另外两组在妊娠第15 - 18天接受亚砷酸钠（10毫克/千克/天）。在接触MMC或亚砷酸盐后，一组接受MMC处理的小鼠和一组接受亚砷酸盐处理的动物立即每天束缚2小时。对照组包括未暴露于MMC或亚砷酸盐的束缚和未束缚的怀孕小鼠。所有动物都被允许分娩并给幼崽断奶。对幼崽进行了身体发育以及行为影响的评估。除了在暴露于亚砷酸盐加束缚的组中，幼崽在出生后第9天的转身行为显著减少外，在未束缚或束缚组中未观察到其他由MMC或亚砷酸盐引起的行为变化。尽管在暴露于亚砷酸盐和束缚的组的幼崽中观察到耳廓分离和眼睛睁开显著延迟，但在接受MMC处理的动物中，发育标志不受束缚的影响。尽管母体应激降低了暴露于MMC加束缚的母鼠的体重增加，但对于发育毒性，无法确定母体应激和MMC之间存在显著的相互作用。这些初步结果，结合先前的研究，表明应激可显著加剧环境有毒元素的不良影响。（C）1997年爱思唯尔科学公司
The present study combined maternal restraint stress with exposure to 2 environmental toxic elements, mercury and arsenic, given to mice concurrently with the restraint period (1000-1200 h, gestational days 15-18). Two groups of animals were given, by gavage, methylmercury chloride (MMC) (2 mg/kg/day), and 2 additional groups received sodium arsenite (10 mg/kg/day) on days 15-18 of gestation. Immediately after MMC or arsenite exposure, 1 group of MMC-treated mice and 1 group of arsenite-treated animals were restrained for 2 h/day. Control groups included restrained and unrestrained pregnant mice nonexposed to MMC or arsenite. All animals were allowed to deliver and wean their offspring. Pups were evaluated for physical development, as well as for behavioral effects. Except for a significant decrease in pivoting on postnatal day 9 in the group exposed to arsenite plus restraint, no other MMC- or arsenite-induced behavioral changes were noted in unrestrained or restrained groups. Although a significant delay in pinna detachment and in eye opening was observed in pups of the group exposed to arsenite and restraint, the developmental landmarks were not affected by restraint in the MMC-treated animals. Although maternal stress reduced body weight gain in the dams exposed to MMC plus restraint, a significant interaction between maternal stress and MMC could not be established for developmental toxicity. These preliminary results, combined with those of previous investigations, show that stress can significantly exacerbate the adverse effects of environmental toxic elements. (C) 1997 Elsevier Science Inc.