AUTOANTIBODIES AGAINST A NOVEL EPITHELIAL CADHERIN IN PEMPHIGUS-VULGARIS, A DISEASE OF CELL-ADHESION
AUTOANTIBODIES AGAINST A NOVEL EPITHELIAL CADHERIN IN PEMPHIGUS-VULGARIS, A DISEASE OF CELL-ADHESION
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DOI：
10.1016/0092-8674(91)90360-b
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发表时间：
1991-11-29
期刊：
影响因子：
64.5
通讯作者：
STANLEY, JR
中科院分区：
文献类型：
--
作者：
AMAGAI, M;KLAUSKOVTUN, V;STANLEY, JR
寻常天疱疮（PV）是一种危及生命的皮肤病，其中针对角质形成细胞表面130 kd糖蛋白（PV抗原（PVA））的自身抗体导致细胞-细胞粘附丧失，从而产生表皮水疱。我们使用亲和纯化的PV IgG从人角质形成细胞表达文库中分离含有PVA的整个编码序列的cDNA。北方印迹分析显示PV mRNA仅在复层鳞状上皮中表达。推导的PVA的氨基酸序列是独特的，但显示出显着的同源性与钙依赖性细胞粘附分子的钙粘蛋白家族的成员，最显着的桥粒芯糖蛋白I。这些发现表明，一种新的上皮钙粘蛋白是PV中自身抗体的靶点。
Pemphigus vulgaris (PV) is a life-threatening skin disease in which autoantibodies against a keratinocyte cell surface 130 kd glycoprotein, PV antigen (PVA), cause loss of cell-cell adhesion, with resultant epidermal blisters. We used affinity-purified PV IgG to isolate CDNA, containing the entire coding sequence for PVA, from human keratinocyte expression libraries. Northern blot analysis indicated PV mRNA expression only in stratified squamous epithelia. The deduced amino acid sequence of PVA was unique but showed significant homology with members of the cadherin family of Ca2+-dependent cell adhesion molecules, most markedly to desmoglein I. These findings demonstrate that a novel epithelial cadherin is the target of autoantibodies in PV.