A multicenter blinded analysis indicates no association between chronic fatigue syndrome/myalgic encephalomyelitis and either xenotropic murine leukemia virus-related virus or polytropic murine leukemia virus.
A multicenter blinded analysis indicates no association between chronic fatigue syndrome/myalgic encephalomyelitis and either xenotropic murine leukemia virus-related virus or polytropic murine leukemia virus.
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作者：
Alter HJ;Mikovits JA;Switzer WM;Ruscetti FW;Lo SC;Klimas N;Komaroff AL;Montoya JG;Bateman L;Levine S;Peterson D;Levin B;Hanson MR;Genfi A;Bhat M;Zheng H;Wang R;Li B;Hung GC;Lee LL;Sameroff S;Heneine W;Coffin J;Hornig M;Lipkin WI
在两项独立的研究中，被称为慢性疲劳综合征或肌痛性脑脊髓炎（CFS/ME）的致残性疾病与异嗜性小鼠白血病病毒相关病毒（XMRV）和多嗜性小鼠白血病病毒（pMLV）的感染有关。尽管这些关联在随后的研究中没有得到其他研究人员的证实，但患者继续质疑科学界拒绝这种关联有效性的共识。在这里，我们报告了盲法分析外周血从一个严格的特点，地理上不同的人口147例CFS/ME和146名健康受试者的研究人员描述的原始关联。该分析未显示XMRV或pMLV感染的证据。慢性疲劳综合征/肌痛性脑脊髓炎在美国的估计患病率为42/10，000，每年的直接医疗费用为70亿美元。在这里，在患有这种疾病的受试者的血液中发现XMRV和pMLV（多嗜性鼠白血病病毒）的原始研究者报告说，这种关联在对严格表征的受试者的样本进行盲法分析中没有得到证实。越来越多地使用分子方法发现病原体，这对公共卫生和科学支持提出了新的挑战。当务之急是制定战略，以快速和连贯地解决发现，使他们可以继续转化为临床医学或放弃更有效地集中资源投资。我们的研究为病原体的发现提供了一个范例，可能有助于该领域的其他工作。
The disabling disorder known as chronic fatigue syndrome or myalgic encephalomyelitis (CFS/ME) has been linked in two independent studies to infection with xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (pMLV). Although the associations were not confirmed in subsequent studies by other investigators, patients continue to question the consensus of the scientific community in rejecting the validity of the association. Here we report blinded analysis of peripheral blood from a rigorously characterized, geographically diverse population of 147 patients with CFS/ME and 146 healthy subjects by the investigators describing the original association. This analysis reveals no evidence of either XMRV or pMLV infection. Chronic fatigue syndrome/myalgic encephalomyelitis has an estimated prevalence of 42/10,000 in the United States, with annual direct medical costs of $7 billion. Here, the original investigators who found XMRV and pMLV (polytropic murine leukemia virus) in blood of subjects with this disorder report that this association is not confirmed in a blinded analysis of samples from rigorously characterized subjects. The increasing frequency with which molecular methods are used for pathogen discovery poses new challenges to public health and support of science. It is imperative that strategies be developed to rapidly and coherently address discoveries so that they can be carried forward for translation to clinical medicine or abandoned to focus resource investment more productively. Our study provides a paradigm for pathogen dediscovery that may be helpful to others working in this field.