Carotenoid accumulations and carotenogenic gene expressions in the petals of Eustoma grandiflorum
Carotenoid accumulations and carotenogenic gene expressions in the petals of Eustoma grandiflorum
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DOI：
10.1111/pbr.12043
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发表时间：
2013-08-01
期刊：
影响因子：
2
通讯作者：
Ohmiya, Akemi
中科院分区：
文献类型：
--
作者：
Liu, Hui;Kishimoto, Sanae;Ohmiya, Akemi
洋桔梗是日本主要的切花之一。深黄色花的品种有市场需求，但从未培育成功。本研究通过对洋桔梗类胡萝卜素积累和类胡萝卜素基因表达的研究，试图探讨洋桔梗深黄色形成受阻的原因。高效液相色谱分析表明，花瓣中类胡萝卜素的组成与叶片中的相似，主要积累叶黄素、紫黄质和β-胡萝卜素。所有供试品种的总类胡萝卜素含量均随花瓣成熟而下降。荧光定量PCR分析表明，PSY、LCYB、ZDS和LCYE在白色和淡黄色花瓣之间以及花瓣和叶片之间的表达量存在显著差异，表明这些酶可能在E.大花在发育过程中，CCD 4在淡黄色和白色花瓣中的表达水平较高，表明花瓣中类胡萝卜素降解活性较高。因此，类胡萝卜素的积累水平取决于类胡萝卜素生物合成和降解活性之间的平衡。
Eustoma grandiflorum is one of the leading cut-flowers in Japan. There are market demands for cultivars with deep-yellow flowers, but they have never been bred successfully. By investigating the carotenoid accumulation and carotenogenic gene expressions, this study attempted to explore the reasons that block the formation of deep-yellow colour in Eustoma. High performance liquid chromatography analysis showed that the carotenoid compositions in petals were similar to those in leaves, accumulating mainly lutein, violaxanthin and beta-carotene. The total carotenoid contents decreased as the petals matured in all the cultivars tested. Quantitative real-time PCR analysis showed that the expression levels of PSY, LCYB, ZDS and LCYE showed significant differences between white and pale-yellow petals or between petals and leaves, indicating that these enzymes may play a key role in the carotenoid biosynthesis in E. grandiflorum. The expression levels of CCD4 were high in both pale-yellow and white petals during development, suggesting that carotenoid degradation activity is high in the petals. We then conclude that the total carotenoid accumulation level could be determined by the balance between carotenoid biosynthesis and degradation activities.