Regulation of Vibrio polysaccharide synthesis and virulence factor production by CdgC, a GGDEF-EAL domain protein, in Vibrio cholerae
Regulation of Vibrio polysaccharide synthesis and virulence factor production by CdgC, a GGDEF-EAL domain protein, in Vibrio cholerae
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DOI：
10.1128/jb.00834-06
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发表时间：
2007-02-01
影响因子：
3.2
通讯作者：
Yildiz, Fitnat H.
中科院分区：
文献类型：
--
作者：
Lim, Bentley;Beyhan, Sinem;Yildiz, Fitnat H.
在霍乱弧菌中，第二信使3 '，5'-环二鸟苷酸（c-di-GMP）调节几种细胞过程，如波纹状菌落形态的形成、生物膜形成、运动性和毒力因子的产生。细胞中c-di-GMP的合成和降解均受含有GGDEF和/或EAL结构域的蛋白质调节，所述蛋白质分别充当二鸟苷酸环化酶和磷酸二酯酶。两个基因，cdgC和mbaA，其编码的蛋白含有GGDEF和EAL结构域的表达是更高的皱纹相变异的霍乱弧菌比光滑的变体。在这项研究中，我们进行了基因表达分析，以确定基因调控的CdgC在皱纹和光滑期变异的V霍乱弧菌。我们确定CdgC调节霍乱弧菌多糖合成所需的基因和转录调节基因vpsR、vpsT和hapR的表达。CdgC还调节参与胞外蛋白分泌、鞭毛生物合成和毒力因子产生的基因的表达。然后，我们比较了由CdgC和MbaA，在指数和静止期的增长，阐明由它们调节的过程中调控的基因。CdgC和MbaA的调节子的鉴定揭示了调节子重叠，但CdgC和MbaA施加的调节时间不同，表明在霍乱弧菌中操作的c-di-GMP信号转导途径的相互作用和复杂性。
In Vibrio cholerae, the second messenger 3',5'-cyclic diguanylic acid (c-di-GMP) regulates several cellular processes, such as formation of corrugated colony morphology, biofilm formation, motility, and virulence factor production. Both synthesis and degradation of c-di-GMP in the cell are modulated by proteins containing GGDEF and/or EAL domains, which function as a diguanylate cyclase and a phosphodiesterase, respectively. The expression of two genes, cdgC and mbaA, which encode proteins harboring both GGDEF and EAL domains is higher in the rugose phase variant of V. cholerae than in the smooth variant. In this study, we carried out gene expression analysis to determine the genes regulated by CdgC in the rugose and smooth phase variants of V cholerae. We determined that CdgC regulates expression of genes required for V. cholerae polysaccharide synthesis and of the transcriptional regulator genes vpsR, vpsT, and hapR. CdgC also regulates expression of genes involved in extracellular protein secretion, flagellar biosynthesis, and virulence factor production. We then compared the genes regulated by CdgC and by MbaA, during both exponential and stationary phases of growth, to elucidate processes regulated by them. Identification of the regulons of CdgC and MbaA revealed that the regulons overlap, but the timing of regulation exerted by CdgC and MbaA is different, suggesting the interplay and complexity of the c-di-GMP signal transduction pathways operating in V. cholerae.