EuroTracker dyes: highly emissive europium complexes as alternative organelle stains for live cell imaging
EuroTracker dyes: highly emissive europium complexes as alternative organelle stains for live cell imaging
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DOI：
10.1039/c3sc53056f
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发表时间：
2014-01-01
期刊：
影响因子：
8.4
通讯作者：
Parker, David
中科院分区：
文献类型：
--
作者：
Butler, Stephen J.;Lamarque, Laurent;Parker, David
已经制备了9种具有不同大环配体的非常明亮的铕（III）配合物，它们表现出优异的细胞摄取行为和独特的亚细胞定位特征，允许使用荧光显微镜和时间门控光谱成像来跟踪它们在细胞中的命运。它们作为细胞成像染色剂的用途描述了对各种哺乳动物细胞类型的线粒体、溶酶体或内质网的选择性照明。
Nine very bright europium(III) complexes with different macrocyclic ligands have been prepared that exhibit excellent cell uptake behaviour and distinctive sub-cellular localisation profiles, allowing the use of fluorescence microscopy and time-gated spectral imaging to track their fate in cellulo. Their use as cellular imaging stains is described for the selective illumination of mitochondria, lysosomes or the endoplasmic reticulum of various mammalian cell types.