Identification and characterization of the inducible murine mast cell gene, imc-415
Identification and characterization of the inducible murine mast cell gene, imc-415
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DOI：
10.1006/bbrc.1998.9609
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发表时间：
1998-11-09
影响因子：
3.1
通讯作者：
Oh, CK
中科院分区：
文献类型：
--
作者：
Cho, SH;Cho, JJ;Oh, CK
肥大细胞的激活导致生物活性介质的产生和释放，进而引发过敏性炎症。刺激后肥大细胞功能得到增强，部分原因是特定基因及其产物的诱导。为了鉴定肥大细胞中诱导的支持这一过程的其他基因，我们构建了一个激活特异性肥大细胞消减文库。迄今为止，我们已经分离出 26 个新型诱导型小鼠肥大细胞 cDNA 克隆。其中，鼠基因imc-415的全编码区与人β(4)整合素结合蛋白(p27(BBP))和人翻译起始因子6(eIF6)具有大于90%的核苷酸序列同源性和97.5%的氨基酸序列同源性，而这两者是相同的。 imc-415 基因的体外翻译产生了大约 26 kDa 的条带。这与根据预测的氨基酸序列计算出的鼠IMC-415蛋白的分子量相同，是p27(BBP)/eIF6的分子量。在哮喘小鼠模型的发炎肺组织中也诱导了小鼠 imc-415 信息。这些结果表明小鼠 imc-415 在过敏性炎症中发挥作用，可能会增强蛋白质合成。基于序列和基因表达模式的相似性，人类 eIF6/p27(BBP) 也可能在过敏性疾病中发挥作用，(C) 1998 年学术出版社。
Activation of mast cells results in the generation and release of bioactive mediators which in turn initiate allergic inflammation. Mast cell function is enhanced following stimulation in part because of the induction of specific genes and their products. To identify additional genes induced in mast cells that support this process, we thus constructed an activation-specific mast cell subtraction library. To date, we have isolated 26 novel inducible murine mast cell time) cDNA clones. Among them, a full-coding region of the murine gene imc-415 was found to have a greater than 90% nueleotide sequence homology and a 97.5% amino acid sequence homology to both a human beta(4) integrin-binding protein (p27(BBP)) and a human translation initiation factor 6 (eIF6), which in turn are identical. In vitro translation of the imc-415 gene yielded a band of an approximately 26 kDa. This is the same as the calculated molecular weight of murine IMC-415 protein based on the predicted amino acid sequence and is the molecular weight of p27(BBP)/eIF6. Murine imc-415 message was also induced in inflamed lung tissues in a mouse model of asthma. These results suggest a role for murine imc-415 in allergic inflammation where it may enhance protein synthesis. Human eIF6/p27(BBP) may also play a role in allergic diseases based on the similarities in sequence and in gene expression patterns, (C) 1998 Academic Press.