The influence in vivo of murine colony-stimulating factor-1 on myeloid progenitor cells in mice recovering from sublethal dosages of cyclophosphamide
The influence in vivo of murine colony-stimulating factor-1 on myeloid progenitor cells in mice recovering from sublethal dosages of cyclophosphamide
复制标题
鼠集落刺激因子1对亚致死剂量环磷酰胺恢复小鼠骨髓祖细胞的体内影响
DOI：
10.1182/blood.v69.3.913.913
复制
发表时间：
1987
期刊：
影响因子：
20.3
通讯作者：
R. Shadduck
中科院分区：
文献类型：
--
作者：
H. Broxmeyer;D. Williams;S. Cooper;A. Waheed;R. Shadduck
纯鼠集落刺激因子-1（CSF-1）的影响进行了评估，在体内的小鼠预处理7天前与亚致死剂量的环磷酰胺。这些小鼠体内的多能祖细胞（CFU-GEMM）、红系祖细胞（BFU-E）和粒细胞-巨噬细胞（CFU-GM）均处于缓慢循环或非循环状态。静脉注射20，000单位的CSF-1刺激这些小鼠的造血祖细胞在3小时内在骨髓和脾脏中进入快速循环状态。骨髓和脾脏CFU-GM、脾脏BFU-E和CFU-GEMM的绝对数量也显著增加。通过鲎溶解物测定在CSF-1制剂中未检测到内毒素，并且在100 ℃下处理CSF-1 20至30分钟完全灭活了体外和体内刺激作用。CSF-1的作用没有通过体内施用0.1至10 ng大肠杆菌脂多糖来模拟。这些结果表明，CSF-1在体内的作用不是由于污染的内毒素或非特异性蛋白质的作用。CSF-1在体外不增强BFU-E的集落形成或刺激CFU-GEMM的集落形成，因此表明CSF-1在体内观察到的至少一些作用可能是间接的，并由辅助细胞介导。
Pure murine colony-stimulating factor-1 (CSF-1) was assessed for its effects in vivo in mice pretreated seven days earlier with a sublethal dosage of cyclophosphamide. The multipotential (CFU-GEMM), erythroid (BFU-E), and granulocyte-macrophage (CFU-GM) progenitor cells in these mice were in a slowly cycling or noncycling state. Intravenous administration of 20,000 units of CSF-1 to these mice stimulated the hematopoietic progenitors into a rapidly cycling state in the marrow and spleen within three hours. Significant increases in absolute numbers of marrow and spleen CFU-GM and spleen BFU-E and CFU-GEMM were also detected. No endotoxin was detected in the CSF-1 preparation by Limulus lysate assay, and treatment of CSF-1 at 100 degrees C for 20 to 30 minutes completely inactivated the in vitro and in vivo stimulating effects. The effects of CSF-1 were not mimicked by the in vivo administration of 0.1 to 10 ng Escherichia coli lipopolysaccharide. These results suggest that the effects of CSF-1 in vivo were not due to contaminating endotoxin or to a nonspecific protein effect. CSF-1 did not enhance colony formation by BFU-E or stimulate colony formation by CFU-GEMM in vitro, thus suggesting that at least some of the effects of CSF-1 noted in vivo are probably indirect and mediated by accessory cells.
登录
查看更多内容
单核吞噬细胞特异性生长因子 CSF-1 与其细胞表面受体的化学交联。
DOI：
10.1016/0006-291x(84)91614-0
发表时间：
1984
影响因子：
3.1
作者：
Morgan,CJ;Stanley,ER
通讯作者：
Stanley,ER
植物血凝素刺激的白细胞条件培养基、氯高铁血红素、前列腺素E和低氧张力对正常人骨髓中红系祖细胞集落形成的比较影响。
影响因子：
2.6
作者：
Lu,L;Broxmeyer,HE
通讯作者：
Broxmeyer,HE
集落刺激因子对扩散室粒细胞生成的调节。
影响因子：
2.6
作者：
Shadduck,RK;Pigoli,G;Waheed,A;Carsten,A;Cronkite,E
通讯作者：
Cronkite,E
骨髓细胞预孵育对集落刺激因子结合的影响。
DOI：
--
发表时间：
1983
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Caramatti,C;Pigoli,G;Shadduck,RK;Waheed,A
通讯作者：
Waheed,A
小鼠腹膜细胞合成骨髓性白血病细胞分化诱导剂 G-CSF：内毒素、M-CSF 和多 CSF 刺激。
影响因子：
2.7
作者：
Metcalf,D;Nicola,NA
通讯作者：
Nicola,NA