Is the ClC-2 chloride channel involved in the Cl- secretory mechanism of gastric parietal cells?
Is the ClC-2 chloride channel involved in the Cl- secretory mechanism of gastric parietal cells?
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DOI：
10.1016/j.febslet.2004.08.044
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发表时间：
2004-09-24
期刊：
影响因子：
3.5
通讯作者：
Sakai, H
中科院分区：
文献类型：
--
作者：
Hori, K;Takahashi, Y;Sakai, H
CIC-2氯离子通道是否参与胃壁细胞的盐酸分泌一直存在争议。在这里，我们研究了 CIC-2 是否是与胃酸分泌相关的顶端 Cl-通道。本研究中使用的两种抗 CIC-2 抗体与 HEK293 细胞中表达的克隆 CIC-2 蛋白发生反应。在离体兔胃腺中，观察到CIC-2 mRNA的显着表达，但CIC-2蛋白的存在尚不清楚。此外，在离体大鼠和人胃粘膜中未观察到CIC-2蛋白的表达。大鼠胃黏膜免疫组化显示壁细胞中CIC-2蛋白无明显表达，而H+,K+-ATP酶大量表达。这些结果表明CIC-2可能不是壁细胞胃酸分泌的Cl-转运蛋白。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
It has been controversial whether the CIC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether CIC-2 is the apical Cl- channel associated with gastric acid secretion. Two anti-CIC-2 antibodies used in this study reacted with cloned CIC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of CIC-2 mRNA was observed, but the presence of CIC-2 protein was not clear. Furthermore, no expression of CIC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of CIC-2 protein in the parietal cells which showed abundant expression of H+,K+-ATPase. These results indicate that CIC-2 may not be a Cl--transporting protein for gastric acid secretion in parietal cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.