Prior beetroot juice ingestion offsets endothelial dysfunction following prolonged sitting
Prior beetroot juice ingestion offsets endothelial dysfunction following prolonged sitting
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事先摄入甜菜根汁可抵消久坐后的内皮功能障碍
DOI：
10.1152/japplphysiol.00200.2022
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发表时间：
2022
影响因子：
3.3
通讯作者：
Eisuke Ochi
中科院分区：
文献类型：
--
作者：
Takuma Morishima;Motoyuki Iemitsu;Shumpei Fujie;Eisuke Ochi
预防久坐后内皮功能障碍的营养策略在很大程度上仍然未知。鉴于甜菜根汁（BRJ）的摄入增强一氧化氮（NO）的生物利用度，我们的目的是评估是否事先BRJ摄入将防止坐在引起的血管内皮功能障碍的腿。11名健康的年轻男性（n= 7）和女性（n= 4）进行了两项实验性试验，长期坐在与先前：1）安慰剂（PL）摄入（PL试验）和2）BRJ摄入（BRJ试验）。所有受试者在坐下前3小时立即摄入140 mL PL或BRJ（分别为0.0055美元或12.8 mmol硝酸盐）。分别在坐位前后测量腘动脉血流介导的舒张功能（FMD）和血压，并采集血液。在坐位期间，每小时评估一次腘动脉直径和血流速度、血压和采血。在两项试验中，在坐位期间腘动脉血流量和剪切率均显著降低，且相似（P< 0.001）。在唯一的BRJ试验中，血浆硝酸盐和NOx（总亚硝酸盐和硝酸盐）浓度相对于基线显著增加，并且BRJ试验中的总体浓度显著更高（P< 0.001）。在PL试验中，坐位后腘动脉FMD显著降低（P< 0.05），而在BRJ试验中未观察到降低。因此，事先BRJ摄入将防止坐引起的腿部内皮功能障碍，通过提高NO biobaicity.NEW &值得注意本研究阐明，甜菜根汁摄入前长时间坐抵消sittinginduced腿部内皮功能障碍。本研究的数据提供了新的生理信息，即通过膳食补充硝酸盐来提高NO的生物利用度是预防长时间坐着对血管有害影响的有效工具。
Nutritional strategies to prevent endothelial dysfunction following prolonged sitting remain largely unknown. Given that beetroot juice (BRJ) ingestion enhances nitric oxide (NO) bioavailability, we aimed to evaluate whether prior BRJ ingestion would prevent sitting-induced endothelial dysfunction in the leg. Eleven healthy young males (n= 7) and females (n= 4) underwent two experimental trials of prolonged sitting with prior: 1) placebo (PL) ingestion (PL trial) and 2) BRJ ingestion (BRJ trial). All subjects ingested 140 mL of PL or BRJ ($0.0055 or $12.8 mmol of nitrate, respectively) immediately before 3 h of sitting. Pre-and postsitting measurements of popliteal artery flow-mediated dilation (FMD) and blood pressure, and blood collection were undertaken. During the sitting period, an hourly assessment of popliteal artery diameter and blood velocity, blood pressure, and blood collection were performed. Popliteal artery blood flow and shear rate were significantly and similarly reduced during the sitting period in both trials (P< 0.001). Plasma nitrate and NOx (total nitrite and nitrate) concentrations were significantly increased relative to baseline in the only BRJ trial, and the overall concentrations were significantly higher in the BRJ trial (P< 0.001). Popliteal artery FMD was significantly reduced after the sitting period in the PL trial (P< 0.05), whereas no reduction was observed in the BRJ trial. Therefore, prior BRJ ingestion would prevent sitting-induced leg endothelial dysfunction via enhancing NO bioavailability.NEW & NOTEWORTHY The present study elucidates that beetroot juice ingestion before prolonged sitting offsets sittinginduced leg endothelial dysfunction. Data from the present study provides novel physiological information that enhancing NO bioavailability by dietary nitrate supplementation is an effective tool for prevention of the detrimental vascular effects of prolonged sitting.