Surface-Modified Electrospun Poly(ε-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction
Surface-Modified Electrospun Poly(ε-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction
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DOI：
10.1167/iovs.13-12727
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发表时间：
2014-02-01
影响因子：
4.4
通讯作者：
Tandon, Radhika
中科院分区：
文献类型：
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作者：
Sharma, Shweta;Gupta, Deepika;Tandon, Radhika
目的。本研究的目的是对合成的聚己内酯(PCL)纳米纤维支架进行表面修饰和功能化处理，以提高其生物相容性，以提供更好的细胞-基质相互作用。采用扫描电子显微镜(SEM)、水接触角测量、拉伸强度和紫外-可见(UV-Vis)分光光度计对支架的形貌、润湿性、机械强度和光学性能进行了表征。通过培养人角膜上皮细胞系(HCE-T)，探讨纳米纤维的生物相容性。随后，培养人角膜缘上皮细胞(LECs)以评价其生物活性。用四甲基偶氮唑蓝(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium))比色法检测细胞增殖。免疫荧光染色和逆转录-聚合酶链式反应检测基因表达；扫描电子显微镜观察细胞形态。结果等离子体处理后的支架形态和拉伸强度与未经处理的支架相似。水接触角测量和光学透明数据表明，与未经处理的PCL支架相比，经等离子体处理的PCL(PPCL)具有更好的润湿性和透明度。生物相容性结果表明，两种支架材料在细胞存活和增殖方面具有良好的生物相容性。而PPCL表现出更好的细胞黏附和增殖能力。结果表明，与未经处理的PCL相比，在PPCL支架上培养的LEC具有更强的细胞黏附和增殖能力。基因表达研究表明，培养物在两种支架上均能保持其正常表型。结论等离子体处理后的支架表面具有亲水性，有效地提高了支架的透明度，促进了支架的生物相容性。这些纳米纤维可以作为支持眼表面工程的生物线索。
PURPOSE. The purpose of this study was to modify and functionalize the surface of synthetic poly-epsilon-caprolactone (PCL) nanofibrous scaffolds to improve their biocompatibility in order to provide better "cell-substrate" interaction.METHODS. Poly-epsilon-caprolactone solution was electrospun and its surface functionality was modified by helium-oxygen (He/O-2) plasma discharge. Scaffolds were characterized for their morphology, wetting ability, mechanical strength, and optical properties by using scanning electron microscopy (SEM), water contact angle measurement, tensile strength, and ultraviolet-visible (UV-Vis) spectrophotometer, respectively. The biocompatibility of nanofibers was explored by culturing human corneal epithelial (HCE-T) cell line. Subsequently, human limbal epithelial cells (LECs) were cultured to evaluate the bioactivity. Cell proliferation was checked by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Immunofluorescent staining and reverse transcription-polymerase chain reaction were done to check the gene expression; SEM was used to study the morphology.RESULTS. Plasma-treated and untreated scaffolds showed almost similar morphology and tensile strength. Water contact angle measurement and optical transparency data showed that the plasma-treated PCL (pPCL) exhibited significantly improved wettability and transparency as compared to the untreated PCL scaffolds. Biocompatibility results indicated that both scaffolds are biocompatible in terms of cell survival and proliferation. However, pPCL showed better cell adhesion and proliferation. Results supported that LEC cultured on pPCL scaffolds had enhanced cell adhesion and proliferation, in comparison to untreated PCL. Gene expression study showed cultures were able to retain their normal phenotype on both scaffolds.CONCLUSIONS. The hydrophilicity of the surface achieved by plasma treatment effectively enhanced the transparency and promoted the biocompatibility of scaffolds. These nanofibers may act as biological cues for endorsing ocular surface engineering.