Arbuscular mycorrhizal fungal communities of a mangrove forest along a salinity gradient on Iriomote Island
Arbuscular mycorrhizal fungal communities of a mangrove forest along a salinity gradient on Iriomote Island
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DOI：
10.1007/s11104-021-05193-4
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发表时间：
2021-10
期刊：
影响因子：
4.9
通讯作者：
Yasuaki Akaji;T. Inoue;T. Taniguchi;S. Baba
中科院分区：
文献类型：
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作者：
Yasuaki Akaji;T. Inoue;T. Taniguchi;S. Baba
丛枝菌根（Arbuscular mycorrhizal，AM）真菌广泛存在于红树林生态系统中，但不同AM真菌与红树林植物的相互作用机制尚不清楚。研究了AM真菌在两种主要红树林树种红海榄（Rhizophora stylosaGriff）根系的定殖规律。和木榄Bruguiera gymnorhiza（L.）拉姆，MethodsWe采样的根和土壤中的一个红树林森林在日本南部的一个岛屿上，并研究了AM真菌的根定殖率和社区组成，使用显微镜和高通量测序的小亚基（18 S）rRNA基因。gymnorhizabut not of R.花柱。分子生物学分析在两种植物根中均检测到球囊霉科植物的DNA序列，而在B.裸胡萝卜球囊霉科植物具有较高的土壤电导率，无梗孢霉科植物具有较低的土壤电导率。存在/不存在的Acaulosporaceae物种的序列是显着相关的存在/不存在的AM真菌结构（显微镜证据）inB。结果表明，盐胁迫影响了红树林土壤AM真菌群落组成，无梗孢菌科等AM真菌与B.在盐碱和低养分环境中，裸根藤可以帮助红树林树体获得营养。
AimsArbuscular mycorrhizal (AM) fungi often occur in mangrove ecosystems, but how different AM fungi interact with mangrove plants is still unclear. Here, we aimed to study the root colonization patterns of AM fungi in two major mangrove tree species,Rhizophora stylosaGriff. andBruguiera gymnorhiza(L.) Lam., that dominate the estuarine and freshwater sides of an ecotone.MethodsWe sampled the roots and soils in a mangrove forest on an island in southern Japan and examined the root colonization rates and community compositions of AM fungi using microscopy and high-throughput sequencing of the small subunit (18S) rRNA gene.ResultsMicroscopy revealed AM fungal structures in roots from some individuals ofB. gymnorhizabut not ofR. stylosa. Molecular analysis detected DNA sequences of Glomeraceae species in roots of both species, but detected those of Acaulosporaceae species only inB. gymnorhizaroots. Glomeraceae species were associated with higher soil electrical conductivity (EC), and Acaulosporaceae species with lower soil EC. The presence/absence of sequences of Acaulosporaceae species was significantly associated with the presence/absence of AM fungal structures (microscopic evidence) inB. gymnorhiza.ConclusionsOur results suggest that salinity influences AM fungal community composition in mangrove soils, and that AM fungi such as Acaulosporaceae species form trophic associations withB. gymnorhizaand could help mangrove tree nutrition in saline and low-nutrient environments.