Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer
Cell Patterning Method on a Clinically Ubiquitous Culture Dish Using Acoustic Pressure Generated From Resonance Vibration of a Disk-Shaped Ultrasonic Transducer
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DOI：
10.1109/tbme.2018.2835834
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发表时间：
2019-01-01
影响因子：
4.6
通讯作者：
Takemura, Kenjiro
中科院分区：
文献类型：
--
作者：
Imashiro, Chikahiro;Kurashina, Yuta;Takemura, Kenjiro
先前已报道过用于细胞培养的细胞图案化方法。然而，这些方法使用一般细胞培养中不使用的内含物或装置，可能会影响细胞功能。在这里，我们报告了一种细胞图案化方法，该方法可以通过利用位于培养皿下方的盘形超声波换能器的共振振动，在没有任何内含物的普通细胞培养皿上进行。单节点圆的共振振动将 C2C12 成肌细胞图案化为培养皿上的圆形，最大峰峰值振幅为 10 μ m(p-p) 的振动暴露 10 分钟。此外，换能器的振幅分布与图案化样品中的细胞密度之间的关系可以表示为线性函数，并且细胞粘附存在明确的振幅阈值。为了评估图案化细胞的细胞功能，我们在图案化后 120 小时培养物中进行了增殖和蛋白质测定。我们的结果表明，细胞增殖率没有降低，细胞蛋白的表达没有变化。因此，我们得出结论，该方法可以成功地对临床上普遍存在的培养皿中的细胞进行图案化，同时保持细胞功能。
Cell patterning methods have been previously reported for cell culture. However, these methods use inclusions or devices that are not used in general cell culture and that might affect cell functionality. Here, we report a cell patterning method that can be conducted on a general cell culture dish without any inclusions by employing a resonance vibration of a disk-shaped ultrasonic transducer located under the dish. A resonance vibration with a single nodal circle patterned C2C12 myoblasts into a circular shape on the dish with 10-min exposure of the vibration with maximum peak-peak amplitude of 10 mu m(p-p). Further-more, the relationship between the amplitude distribution of the transducer and the cell density in the patterned sample could be expressed as a linear function, and there was a clear threshold of amplitude for cell adhesion. To evaluate the cell function of the patterned cells, we conducted proliferation and protein assays at 120-h culture after patterning. Our results showed that the cell proliferation rate did not decrease and the expression of cellular proteins was unchanged. Thus, we conclude, this method can successfully pattern cells in the clinically ubiquitous culture dish, while maintaining cell functionality.