Ouabain stimulation of 45 CA ++ accumulation in the rat pituitary.
Ouabain stimulation of 45 CA ++ accumulation in the rat pituitary.
复制标题
哇巴因刺激大鼠垂体中 45 CA 的积累。
DOI：
10.1210/endo-92-5-1555
复制
发表时间：
1973
期刊：
影响因子：
4.8
通讯作者：
J. Wood
中科院分区：
文献类型：
--
作者：
N. Fleischer;J. Wood
Anterior pituitary tissue from rats was incubated at 37 C in Krebs' Ringer's bicarbonate in an atmosphere of 95% O2 and 5% CO2 with hourly changes of the incubation media. After a one hour preincubation, 45Ca++ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 X 10-4 and 1 X 10-3M significantly augmented pituitary 45Ca++. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for 14C inulin or affect pituitary weights suggesting that the observed changes in 45Ca++ content were the result of increased 45Ca++ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain. (Endocrinology 92: 1555, 1973)
