Effect of postharvest ethylene treatment on sugar content, glycosidase activity and its gene expression in mango fruit.
Effect of postharvest ethylene treatment on sugar content, glycosidase activity and its gene expression in mango fruit.
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DOI：
10.1002/jsfa.7912
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发表时间：
2017-03-01
影响因子：
4.1
通讯作者：
Gupta, Vidya S
中科院分区：
文献类型：
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作者：
Chidley, Hemangi G;Deshpande, Ashish B;Gupta, Vidya S
背景：成熟相关的软化是很大程度上决定芒果（Mangifera indica Linn.）果实保质期的重要属性之一。为了揭示更年期前乙烯处理对阿方索芒果成熟相关软化的影响，对成熟的阿方索生果进行了乙烯处理。分析了对照和乙烯处理果实在成熟过程中还原性和非还原性糖池的变化、β -d-半乳糖苷酶、α -d-甘露糖苷酶和β -d-葡萄糖苷酶三种糖苷酶的酶活性及其相对转录物丰度。结果：乙烯处理后，三种糖苷酶活性明显提早，还原糖和非还原糖积累加速。对照果实中-d-半乳糖苷酶活性最高，乙烯处理后活性显著增加。通过对其在对照和乙烯处理的成熟果实中活性的组织化学分析证实了这一点。相对转录丰度表明对照果实中β -d-半乳糖苷酶的转录水平较高。乙烯处理的果实中β -d-半乳糖苷酶转录体较早显著增加，α -d-甘露糖苷酶转录体变异较早积累。结论：经绝经前乙烯处理后，阿方索芒果的保质期缩短，β -d-半乳糖苷酶和α -d-甘露糖苷酶在阿方索果实成熟相关软化过程中发挥了重要作用，并通过乙烯转录调控了它们的表达。©2016化学工业协会。
BACKGROUND: Ripening-associated softening is one of the important attributes that largely determines the shelf-life of mango (Mangifera indica Linn.) fruits. To reveal the effect of pre-climacteric ethylene treatment on ripening-related softening of Alphonso mango, ethylene treatment was given to mature, raw Alphonso fruits. Changes in the pool of reducing and non-reducing sugars, enzymatic activity of three glycosidases: beta-d-galactosidase, alpha-d-mannosidase and beta-d-glucosidase and their relative transcript abundance were analysed for control and ethylene treated fruits during ripening.RESULTS: Early activity of all the three glycosidases and accelerated accumulation of reducing and non-reducing sugars on ethylene treatment was evident. beta-d-Galactosidase showed the highest activity among three glycosidases in control fruits and marked increase in activity upon ethylene treatment. This was confirmed by the histochemical assay of its activity in control and ethylene treated ripe fruits. Relative transcript abundance revealed high transcript levels of beta-d-galactosidase in control fruits. Ethylene-treated fruits showed early and remarkable increase in the beta-d-galactosidase transcripts while alpha-d-mannosidase transcript variants displayed early accumulation.CONCLUSION: The findings suggest reduction in the shelf-life of Alphonso mango upon pre-climacteric ethylene treatment, a significant role of beta-d-galactosidase and alpha-d-mannosidase in the ripening related softening of Alphonso fruits and transcriptional regulation of their expression by ethylene. © 2016 Society of Chemical Industry.